Objective: To observe the action mechanism of components of water extract from Qixue Bingzhi recipe(CWQB) for adenosine monophosphate activated protein kinase (AMPK) related glucose metabolism and fatty acid oxidation pathways in hypoxia/reoxygenation(H/R) myocardial cells.Method: The myocardial cells from 1-2 d newborn SD rats were separated and extracted. Morphology of primary cardiacmyocyte under different duration of culture was observed by an optical microscope. After α-actinin immunofluorescence identified cardiomyocytes

the H/R models were made by depriving oxygen for 3 hours and then regaining oxygen for 2 hours. Then the cardiomyocytes were randomly divided into four groups:control group (with normal oxygen)

H/R group(the H/R model)

trimetazidine (TMZ) group

and CWQB group.The H/R cardiomyocytes in TMZ group were treated by TMZ 100 μmol · L-1; H/R cardiomyocytesin CWQB group were treated by CWQB 1 μmol · L-1. Finally

the mRNA and proteinexpressions of adenosine monophosphate activated protein kinase-α (AMPKα) together with its related glucose metabolism pathway including glucose transporter4 (GLUT4) and phosphate fructose kinase 2 (PFK2)

fatty acid metabolism pathway including fatty acid translocase/CD36(FAT/CD36) and acetyl-CoA carboxylase 2 (ACC2) were figured out by Real-time polymerase chain reaction(Real-time PCR)and Western blot.Result: As compared with the control group

both mRNA and proteinexpressions of AMPKα

GLUT4

PFK2 were increased

while ACC2

FAT/CD36 mRNA and proteinexpressions were decreased in H/R group

TMZ group

and CWQB group (P<0.05). As compared with H/R group

the mRNA and proteinexpressions of AMPKα

GLUT4

PFK2

ACC2 and FAT/CD36 were increased significantly in TMZ group and CWQB group (P<0.05). By the way

the up-regulation of mRNA and protein expressionsof AMPKα and FAT/CD36

as well as mRNA expressions of GLUT4 and PFK2 in TMZ group were more obvious than those in CWQB group(P<0.05).Conclusion: CWQB can activate the AMPK pathway in H/R cardiomyocytes

enhance glucose transport mediated by GLUT4 and glycolysis mediated by PFK2

promote the absorption of fatty acid adjusted by FAT/CD36

and inhibit its oxidation by up-regulating ACC2.Therefore

CWQB recipe can improve the utilization of energy materials in myocardial cells under H/R situation

and then optimize the energy metabolism to prevent damages caused by energy deficiency.