Effect of Liquiritin on UVB-induced Apoptosis of Human Keratinocytes

ZHANG Li-hong; FU Yun; LIAO Jian; ZHANG Qiong; ZHOU Li-lu; ZHOU Li; WANG Ye-qiu; LI Jian-min

doi:10.13422/j.cnki.syfjx.20180696

您当前的位置：

首页 >

文章列表页 >

Effect of Liquiritin on UVB-induced Apoptosis of Human Keratinocytes

更新时间：2024-01-04

- Effect of Liquiritin on UVB-induced Apoptosis of Human Keratinocytes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 6, Pages: 96-101(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20180696
  CLC： R285
- Published：2018
- 稿件说明：
移动端阅览
ZHANG Li-hong, FU Yun, LIAO Jian, et al. Effect of Liquiritin on UVB-induced Apoptosis of Human Keratinocytes[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 96-101.
DOI：

ZHANG Li-hong, FU Yun, LIAO Jian, et al. Effect of Liquiritin on UVB-induced Apoptosis of Human Keratinocytes[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 96-101. DOI： 10.13422/j.cnki.syfjx.20180696.

摘要

目的：研究甘草苷对中波紫外线（UVB）诱导人皮肤角质形成细胞（HaCaT）凋亡的影响。方法：以不同浓度的甘草苷作用于HaCaT细胞，噻唑蓝（MTT）比色法筛选甘草苷的有效安全浓度；用30 mJ · cm-2的UVB作用于HaCaT细胞，建立光老化模型，MTT比色法检测光老化HaCaT细胞增殖率；流式细胞术检测各组细胞中活性氧自由基（ROS）含量和总凋亡率；实时荧光定量PCR （Real-time PCR）检测光老化HaCaT细胞中半胱氨酸天冬氨酸-3（Caspase-3），半胱氨酸天冬氨酸-9（Caspase-9） mRNA表达水平；蛋白免疫印记法（Western blot）检测光老化HaCaT细胞中B淋巴细胞瘤-2（Bcl-2），Caspase-3，Caspase-9蛋白表达量。结果：筛选出甘草苷的最佳有效安全浓度为1×10-7，1×10-6，1×10-5mol · L-1。与空白组比较，UVB组细胞增殖率显著降低（P<0.01），细胞中ROS含量和总凋亡率显著升高（P<0.01），Caspase-3，Caspase-9 mRNA表达水平显著升高（P<0.01）；Bcl-2蛋白表达量显著降低（P<0.01），Caspase-3，Caspase-9蛋白表达量显著升高（P<0.01）。与UVB组比较，1×10-7，1×10-6，1×10-5mol · L-1甘草苷+UVB组细胞增殖率显著升高（P<0.01）；细胞中ROS含量和总凋亡率显著降低（P<0.05，P<0.01），Caspase-3，Caspase-9 mRNA表达水平显著降低（P<0.05，P<0.01）；Bcl-2蛋白表达量显著升高（P<0.05，P<0.01），Caspase-3，Caspase-9蛋白表达量显著降低（P<0.05，P<0.01）。结论：甘草苷能通过促进抗凋亡因子Bcl-2蛋白的表达，减少促凋亡相关细胞因子Caspase-3，Caspase-9 mRNA和蛋白的表达，从而抑制UVB诱导的HaCaT细胞凋亡。

Abstract

Objective: To investigate the effects of liquiritin on ultraviolet B (UVB)-induced apoptosis of human keratinocytes (HaCaT cells).Method: Different concentrations of liquiritinwere used to treat HaCaT cells

and the methyl thiazolyltetrazolium(MTT) assay was used to screen the effective and safe concentration of liquiritin;light aging model was set up by the irradiation of ultraviolet radiation B (UVB) at irradiation dose of 30 mJ · cm-2. Then the activity of cells was determined by MTT method;the content of reactive oxygen species (ROS) and the apoptosis of HaCaT cells were respectively measured by flow cytometry;the expression levels of cysteinyl aspartate-3(Caspase-3) and cysteinyl aspartate-9(Caspase-9) mRNA were determined by Real-time PCR method; and Western blot method was used to detect the effects of liquiritin on B-cell lymphoma 2(Bcl-2)

Caspase-3 and Caspase-9 protein expression.Result: The optimal safe and effective concentrations of liquiritin were 1×10-7

1×10-6

and 1×10-5 mol · L-1. As compared with the blank group

the cell proliferation rate was significantly decreased in the UVB group (P<0.01);the content of ROS and the total apoptosis rate of cells were significantly increased(P<0.01); the expression levels of Caspase-3 and Caspase-9 mRNA were significantly increased (P<0.01);the expression level of Bcl-2 protein was significantly decreased (P<0.01)

and the expression levels of Caspase-3 and Caspase-9 protein were significantly increased (P<0.01). As compared with the UVB group

the cell proliferation rate of the (1×10-7

1×10-6

and 1×10-5) mol · L-1liquiritin+UVB groups were significantly increased (P<0.01);the content of ROS and the total apoptosis rate of cells were significantly decreased (P<0.05

P<0.01); the expression levels of Caspase-3 and Caspase-9 mRNA were significantly decreased(P<0.05

P<0.01);the expression level of Bcl-2 protein was significantly increased(P<0.05

P<0.01)

and the expression levels of Caspase-3 and Caspase-9 protein were significantly decreased (P<0.05

P<0.01).Conclusion: Liquiritin can promote the expression of anti-apoptotic factor Bcl-2 protein

and decrease the expression of the apoptosis-related factors Caspase-3 and Caspase-9 mRNA and protein

thus inhibiting the apoptosis of HaCaT cells induced by UVB.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Baicalein on Ultraviolet B-induced Apoptosis and Expression of Related Cytokines of Human Keratinocytes

Treatment of Sepsis-induced Inflammatory Responses with Xijiao Dihuangtang by Modulation of PKM2-mediated One-carbon Metabolism Pathway

Mitochondrial Quality Control Regulating Pathogenesis of Sarcopenia and Its Intervention by Traditional Chinese Medicine： A Review

Mechanism of Modified Danggui Shaoyaosan in Improving Inflammation and Apoptosis in Acne via Regulating JNK/p38 MAPK Pathway

Programmed Cell Death in Endometriosis and Traditional Chinese Medicine Intervention： A Review

Related Author

WANG Dong-mei

YAN Qixiang

ZHU Yeyan

GE Fan

SUN Qimeng

YE Leyao

TIAN Fang

LU Jun

Related Institution

Affiliated Hospital of Nanjing University of Chinese Medicine

The First Clinical Medical College，Nanjing University of Chinese Medicine

Jiangsu Province Hospital of Chinese Medicine

The First Affiliated Hospital of Henan University of Chinese Medicine

The First Clinical Medical School of Henan University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰