Chemical Constituents from Stems of

XIONG Hui; TU Chu-yue; JIANG Hai-qin; WU Xin-yan; HE Si-wen; MEI Zhi-nan

doi:10.13422/j.cnki.syfjx.20180713

您当前的位置：

首页 >

文章列表页 >

Chemical Constituents from Stems of

更新时间：2024-01-04

- Chemical Constituents from Stems of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 8, Pages: 49-53(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20180713
  CLC： R284.1
- Published：2018
- 稿件说明：
移动端阅览
XIONG Hui, TU Chu-yue, JIANG Hai-qin, et al. Chemical Constituents from Stems of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 49-53.
DOI：

XIONG Hui, TU Chu-yue, JIANG Hai-qin, et al. Chemical Constituents from Stems of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 49-53. DOI： 10.13422/j.cnki.syfjx.20180713.

摘要

目的：过岗龙为植物榼藤Entada phaseoloides的干燥藤茎，民间常用于风湿骨痛的治疗，为探寻过岗龙的化学物质和活性组分，课题组对其化学成分进行系统研究。方法：取过岗龙9.0 kg，粉碎后用70%乙醇室温浸提3次，每次24 h，提取液减压浓缩，加水混悬后，依次用石油醚、乙酸乙酯和正丁醇萃取，利用正反相硅胶，LH-20羟丙基葡聚糖凝胶（Sephadex LH-20），小孔树脂凝胶（MCI）等柱色谱和半制备高效液相色谱对正丁醇部位进行分离纯化，应用1H-NMR，13 C-NMR和MS等现代波谱分析技术结合理化性质鉴定所得化合物的结构。结果：从过岗龙70%乙醇提取物中分离得到10个化合物，分别鉴定为没食子儿茶素（1），尿嘧啶核苷（2），表没食子儿茶素（3），儿茶素（4），表儿茶素（5），原花青素B3（6），原花青素B1（7），圣草次苷（8），（-）-香橙素-3-O-β-D-吡喃葡萄糖苷（9）和柚皮苷二氢查尔酮（10）。结论：化合物2，6~10为首次从该植物中分离得到。

Abstract

Objective: The stems of Entada phaseoloides have been used for the treatment of rheumatoid arthritis and traumatic injuries. In order to explore its bioactive constituents

70% ethanol extract was studied systemically in chemistry. Method: The dried and powdered stems (9 kg) of E. phaseoloides were extracted for tree times with 70% ethanol at room temperature (3×24 h). The combined ethanol extracts were extracted consecutively with petroleum ether

EtOAc and n-BuOH. The components of n-BuOH extract were isolated and purified by normal and reverse phase silica gels

Sephadex LH-20 and MCI column chromatography and semi-preparative HPLC. And their structures were confirmed based on the physicochemical properties and spectral analyses. Result: Ten compounds were isolated from the n-BuOH fraction of 70% ethanol extract from stems of E. phaseoloides

which were identified as epigallocatechin (1)

uridine (2)

gallocatechin (3)

catechin (4)

epicatechin (5)

procyanidin B3 (6)

procyanidin B1 (7)

eriocitrin (8)

(-)-aromadendrin-3-O-β-D-glucopyranoside (9) and naringin dihydrochalcone (10). Conclusion: Compounds 2 and 6-10 were isolated from this plant for the first time.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

In Vitro and in vivo Component Identification of Danshenyin Based on UPLC-Q-TOF-MS/MS

Editorial Explanation of Guidelines for Establishing Animal Models of Rheumatoid Arthritis with Cold-dampness Obstruction Syndrome and Dampness-heat Obstruction Syndrome

Guidelines for Establishing Animal Models of Rheumatoid Arthritis with Cold-dampness Obstruction Syndrome and Dampness-heat Obstruction Syndrome

Clinical Characteristics and Influencing Factors of Rheumatoid Arthritis in Patients with Cold Dampness Obstruction Syndrome

Identification of in Vitro and in vivo Chemical Constituents of Ruyi Zhenbaowan Based on UHPLC-Q Exactive Orbitrap HRMS

Related Author

ZHANG Sitong

HU Xianrun

LIU Wenkang

LEI Jinchun

CHENG Xuemei

WU Xiaojun

CHEN Wansheng

LI Manlin

Related Institution

Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Southern Medical University Hospital of Integrated Chinese and Western Medicine

Tongji Hospital， Tongji Medical College of Huazhong University of Science and Technology

School of Chinese Materia Medica， Beijing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰