Effect and Mechanism of Longdan Xiegan Tang on Polycystic Ovarian Syndrome Model Rats

LI Jun-bo; DENG Fang; LI Xiao-han; XU Li-mian

doi:10.13422/j.cnki.syfjx.20180725

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Effect and Mechanism of Longdan Xiegan Tang on Polycystic Ovarian Syndrome Model Rats

更新时间：2024-01-04

- Effect and Mechanism of Longdan Xiegan Tang on Polycystic Ovarian Syndrome Model Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 7, Pages: 121-126(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20180725
  CLC： R-332;R285.5
- Published：2018
- 稿件说明：
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LI Jun-bo, DENG Fang, LI Xiao-han, et al. Effect and Mechanism of Longdan Xiegan Tang on Polycystic Ovarian Syndrome Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 121-126.
DOI：

LI Jun-bo, DENG Fang, LI Xiao-han, et al. Effect and Mechanism of Longdan Xiegan Tang on Polycystic Ovarian Syndrome Model Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 121-126. DOI： 10.13422/j.cnki.syfjx.20180725.

摘要

目的：研究龙胆泻肝汤对多囊卵巢综合征（PCOS）模型大鼠药效观察及机制，为龙胆泻肝汤临床PCOS的应用提供初步实验依据。方法：将SD大鼠50只，随机分为2组，即正常组10只、模型制备组40只，采用胰岛素（INS）联合人绒毛膜促性腺激素（HCG）法复制大鼠PCOS模型，造模成功的大鼠分为模型组、达英-35（0.21 g · kg-1）及龙胆泻肝汤高、低剂量组（29.6，7.4 g · kg-1），除正常组外，其余各组连续灌胃给药15 d；动物处理后测量卵巢质量及体积大小，检测各组大鼠血清中促黄体生成素（LH）/卵泡刺激素（FSH），睾酮（T），空腹血糖，空腹胰岛素（INS）激素水平及炎症因子白细胞介素-2（IL-2），IL-4，IL-6，IL-10水平；蛋白免疫印迹法（Western blot）分析各组卵巢组织中JAK2，STAT3，p-STAT3和IL-6蛋白的表达量。结果：与正常组比较，模型组卵巢质量及体积明显升高，血清LH/FSH，T，空腹血糖及INS均明显升高，IL-2，IL-6，IL-10水平明显升高，JAK2，STAT3和IL-6蛋白的表达量明显升高（P<0.05）；与模型组比较，龙胆泻肝汤高、低剂量组明显降低卵巢质量及体积，血清LH/FSH，T，空腹血糖及INS，IL-2，IL-6，IL-10水平，升高JAK2，STAT3蛋白的表达量，降低p-STAT3和IL-6蛋白的表达量（P<0.05）。结论：龙胆泻肝汤对PCOS大鼠具有明显疗效，主要通过降低激素水平和抑制炎症反应；且其机制可能与JAK2/STAT3通路相关。

Abstract

Objective: To study the therapeutic effect and mechanism of Longdan Xiegan Tang on polycystic ovary syndrome (PCOS) model and provide a preliminary experimental basis for the application of PCOS. Method: Totally 50 SD rats were randomly divided into normal group (n=10) and modeling group (n=40). PCOS models were established by using insulin (INS) and human chorionic gonadotropin (HCG). The successfully modeled rats were then divided into model group

Daying-35 group (0.21 g · kg-1)

Longdan Xiegan Tang high-dose group (29.6 g · kg-1) and low-dose group (7.4 g · kg-1). After continuous intragastric administration of 15 d

we analyzed the ovarian weight and volume. The levels of luteinizing hormone (LH)/follicle stimulating hormone (FSH)

testosterone (T)

fasting blood glucose

fasting insulin (INS) and levels of inflammatory cytokines interleukin (IL)-2

IL-4

IL-6 and IL-10; Western blot was used to analyze protein expression of JAK2

STAT3

p-STAT3 and IL-6 in ovarian tissues. Result: As compared with the normal group

ovarian weight and volume were significantly increased; LH/FSH

fasting blood glucose and INS levels in serum were significantly increased; IL-2

IL-6 and IL-10 levels were significantly increased; and JAK2

STAT3 and IL-6 protein expression levels were also up-regulated in model group (P<0.05). As compared with the model group

high dose and low dose Longdan Xiegan Tang significantly decreased ovarian weight and volume

LH/FSH

fasting blood glucose and INS levels in serum as well as IL-2

IL-6 and IL-10 levels

increased JAK2

STAT3 protein expression and reduced p-STAT3 and IL-6 protein expression (P<0.05). Conclusion: Longdan Xiegan Tang has obvious curative effect on PCOS rats by decreasing hormone level and inhibiting inflammatory reaction and the mechanism may be related to JAK2/STAT3 pathway.

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