Protective Effect of Shenfu Injection on Myocardium of Rats with Severe Acute Pancreatitis

YANG Wei-qin; LI Pei-wu; MI Hong; ZHAO Di-ke; XIANG Chang-qin; CHEN Xu; LIU Feng-bin

doi:10.13422/j.cnki.syfjx.20180727

您当前的位置：

首页 >

文章列表页 >

Protective Effect of Shenfu Injection on Myocardium of Rats with Severe Acute Pancreatitis

更新时间：2024-01-04

- Protective Effect of Shenfu Injection on Myocardium of Rats with Severe Acute Pancreatitis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 7, Pages: 159-165(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20180727
  CLC： R285.5
- Published：2018
- 稿件说明：
移动端阅览
YANG Wei-qin, LI Pei-wu, MI Hong, et al. Protective Effect of Shenfu Injection on Myocardium of Rats with Severe Acute Pancreatitis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 159-165.
DOI：

YANG Wei-qin, LI Pei-wu, MI Hong, et al. Protective Effect of Shenfu Injection on Myocardium of Rats with Severe Acute Pancreatitis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 159-165. DOI： 10.13422/j.cnki.syfjx.20180727.

摘要

目的：探讨参附注射液治疗重症急性胰腺炎模型大鼠心肌的保护作用。方法：选取雄性SD大鼠60只，随机分为6组，分别为假手术组、模型组、乌司他丁组（2×104 U · kg-1）和参附注射液高、中、低剂量组（3，1.5，0.75 g · kg-1）。实验采用逆行胆胰管灌注5%牛磺胆酸钠溶液复制重症急性胰腺炎大鼠模型，造模后3 h，分别给予尾静脉注射生理盐水、乌司他丁、不同剂量参附注射液。6 h后苏木素-伊红（HE）染色法观察各组大鼠胰腺及心肌的病理变化，并检测大鼠心肌细胞Na+-K+-ATP酶活性，实时荧光定量聚合酶链式反应（qPCR）法检测肿瘤坏死因子-α（TNF-α），白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）mRNA表达量。结果：与假手术组比较，模型组的大鼠胰腺、心肌损伤严重，病理评分明显升高（P<0.05），心肌Na+-K+-ATP酶活性明显降低（P<0.05），TNF-α，IL-1β mRNA表达量明显升高（P<0.05）；与模型组比较，乌司他丁组、参附注射液组的胰腺和心脏病理评分明显降低（P<0.05），参附注射液组大鼠心肌Na+-K+-ATP酶活性明显增加（P<0.05），TNF-α，IL-1β mRNA表达量明显降低（P<0.05）。结论：参附注射液可能通过改善心肌细胞Na+-K+-ATP酶活性，减少心肌组织中TNF-α，IL-1β的表达，对心肌起保护作用。

Abstract

Objective: To investigate the protective effect and mechanism of Shenfu injection on myocardium in rats with severe acute pancreatitis. Method: Totally 60 male SD rats were randomly divided into 6 groups:Sham operation group

model group

ulinastatin (2×104 U · kg-1) group and Shenfu injection groups (3

1.5

0.75 g · kg-1). The models of severe acute pancreatitis were established by retrograde cholangiopancreatic injection of 5% sodium taurocholate solution. Three hours after modeling

the normal saline

ulinastatin and Shenfu injection were respectively given by tail vein injection. After 6 hours

the pathological changes of pancreas and myocardium were observed; the expression of Na+-K+-ATPase activity was detected; and the mRNA expression levels of tumor necrosis factor-α (TNF-α)

interleukin-1β (IL-1β) and interleukin-6 (IL-6) in rat cardiomyocytes were detected by Real-time fluorescent quantitative polymerase chain reaction (qPCR). Result: As compared with the sham operation group

the pancreatic and myocardial injuries were severe and pathological score was significantly increased in model group (P<0.05); the activity of Na+-K+-ATPase was significantly decreased (P<0.05)

and the mRNA expression levels of TNF-α and IL-1β were significantly increased (P<0.05). As compared with the model group

the pancreatic and cardiac pathological scores were significantly decreased in Ulinastatin group and Shenfu injection groups (P<0.05); the activity of Na+-K+-ATPase in the myocardium was significantly increased and the mRNA expression levels of TNF-α and IL-1β were significantly decreased in Shenfu injection group (P<0.05). Conclusion: Shenfu injection may improve the activity of Na+-K+-ATPase in myocardial cells and decrease the mRNA expression of TNF-α and IL-1β in myocardium to protect the myocardium.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Protective Effect of Xuebijing on Lung Injury in Rats with Severe Acute Pancreatitis by Blocking FPRs/NLRP3 Inflammatory Pathway

Effect of Reduning Injection， Shenfu Injection Combined with Gefitinib on Proliferation， Stemness Characteristics and Metabolism of Non-small Cell Lung Cancer Cells

Xuebijing Injection Regulates Mitochondrial N-formyl Peptides/NLRP3 Inflammatory Pathway to Treat Severe Acute Pancreatitis in Rats

Clinical Efficacy of Modified Qingyitang Combined with Blood Purification on Severe Acute Pancreatitis Complicated with Multiple Organ Dysfunction Syndrome

Clinical Efficacy of Dahuang Zhuyu Decoction for Oral Administration and Enema on Severe Acute Pancreatitis with Syndromes of Blood Stasis, Toxin and Its Effect on Serum Inflammatory Factors

Related Author

ZHANG Guixian

LIU Dawei

LI Xia

LI Xijing

SHI Pengcheng

FENG Zhiqiao

CAI Jun

ZONG Wenhui

Related Institution

Health Commission of Heping District

Tianjin Chase Sun Pharmaceutical Co. Ltd.

Tianjin Institute of Medical and Pharmaceutical Science

The Second Clinical College of Guangzhou University of Chinese Medicine

HongKong-Macao Joint Lab on Chinese Medicine and Immune Disease Research

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰