Effect of Qinyu Sanlong Decoction on Expression of SFRP-2 Antagonistic Wnt Signal Pathway

ZHANG Xing-xing; LI Ze-geng; ZENG Shi-jie; ZHANG Zhi-qiang; HU Jian

doi:10.13422/j.cnki.syfjx.20180793

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Effect of Qinyu Sanlong Decoction on Expression of SFRP-2 Antagonistic Wnt Signal Pathway

更新时间：2024-01-04

- Effect of Qinyu Sanlong Decoction on Expression of SFRP-2 Antagonistic Wnt Signal Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 7, Pages: 127-132(2018)
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- DOI：10.13422/j.cnki.syfjx.20180793
  CLC： R285.5
- Published：2018
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ZHANG Xing-xing, LI Ze-geng, ZENG Shi-jie, et al. Effect of Qinyu Sanlong Decoction on Expression of SFRP-2 Antagonistic Wnt Signal Pathway[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 127-132.
DOI：

ZHANG Xing-xing, LI Ze-geng, ZENG Shi-jie, et al. Effect of Qinyu Sanlong Decoction on Expression of SFRP-2 Antagonistic Wnt Signal Pathway[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 127-132. DOI： 10.13422/j.cnki.syfjx.20180793.

摘要

目的：探讨中药复方芪玉三龙汤（Qinyu Sanlong decoction）对Wnt（Wingless，Int1的合称）信号通路拮抗基因分泌型Frizzled相关蛋白（SFRP）-2的影响。方法：基于前期研究基础，采用LLC细胞培养移植法，构建小鼠移植肺癌模型，将荷瘤小鼠随机分为模型组、芪玉三龙汤组（低、中、高剂量组）、化疗组、联合组、芪玉三龙汤组，小鼠分别按20.12，40.24，80.48 g ·kg-1 ·d-1灌胃给药，化疗组以顺铂0.4 mL腹腔注射给药（1次/周），联合组以芪玉三龙汤80.48 g ·kg-1 ·d-1灌胃和腹腔注射顺铂联合给药，模型组以等体积0.9%氯化钠溶液灌胃，1次/d，连续给药21 d；称取瘤质量，计算抑瘤率；透射电镜观察肿瘤细胞超微结构；蛋白免疫印迹法（Western blot）检测肿瘤组织β-链蛋白（β-catenin），SFRP-2蛋白表达。结果：与模型组相比，各用药组均可下调β-catenin蛋白表达（P<0.01）；与芪玉三龙汤20.12 g ·kg-1 ·d-1组比较，其他各用药组亦可下调β-catenin蛋白表达（P<0.01）；与化疗组比较，芪玉三龙汤40.24，80.48 g ·kg-1 ·d-1组、联合组下调较明显（P<0.05，P<0.01）。与模型组相比，芪玉三龙汤40.24，80.48 g ·kg-1 ·d-1组均可促进SFRP-2蛋白表达（P<0.05，P<0.01）；与芪玉三龙汤20.12 g ·kg-1 ·d-1组比较，其他各用药组亦可上调SFRP-2蛋白表达水平（P<0.01）；与化疗组比较，芪玉三龙汤40.24，80.48 g ·kg-1 ·d-1组均可促进SFRP-2蛋白表达（P<0.05，P<0.01）。用药组电镜下可见凋亡现象，以芪玉三龙汤80.48 g ·kg-1 ·d-1组、化疗组及联合组明显。结论：芪玉三龙汤可一定程度抑制肺癌生长，诱导其镜下凋亡，促进SFRP-2表达，从而抑制Wnt/β-catenin信号通路。

Abstract

Objective: To explore the effects of Qinyu Sanlong decoction on expression of secreted frizzled-related proteins (SFRP)-2 antagonistic Wnt signal pathway. Method: Based on the previous research

LLC cells were used to construct lung cancer model. The mice bearing tumor were divided into model group (M)

Qinyu Sanlong decoction groups (low

middle and high dose groups

named as QL

QH respectively)

chemotherapy group (C)

and combination group (CQH). Qinyu Sanlong decoction groups were administrated with 20.12

40.24

and 80.48 g · kg-1 · d-1 by gavage. C group was given with 0.4 mL cisplatin by intraperitoneal injecting. CQH group was administered with high dosage of Qinyu Sanlong decoction by gavage and cisplatin by intraperitoneal injecting. M group was administered with the same amount of 0.9% sodium chloride solution

once a day for 21 d. The tumors were weighed to calculate the tumor inhibition rate. Transmission electron microscopy was used to observe the ultrastructure of tumor. The SFRP-2 and β-catenin protein expression levels in tumor tissues were detected by Western blot. Result: As compared with M group

every medication group could down-regulate β-catenin expression (P<0.01); as compared with QL group

other medication groups also could down-regulate β-catenin expression (P<0.01); as compared with C group

the down-regulation was more obvious in QM group

QH group and CQH group (P<0.05

P<0.01). The results of SFRP-2 showed that SFRP-2 expression was up-regulated in QM and QH groups as compared with M group (P<0.05

P<0.01); every other medication group also could up-regulate SFRP-2 expression as compared with QL group (P<0.01); QM and QH groups could up-regulate SFRP-2 expression as compared with C group (P<0.05

P<0.01). Also

apoptosis was found under electron microscope in medication groups

especially in QH

C and CQH groups. Conclusion: Qinyu Sanlong decoction can effectively inhibit tumor growth

induce apoptosis

promote the SFRP-2 expression and inhibit the Wnt/β-catenin signal pathway.

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