Effect of Extraction and Processing on Chemical Constituents in Bupleuri Radix by HPLC-ELSD Fingerprint

CONG Meng-yu; GONG Yan-rong; LIANG Sha-bi; NI Jie-na; WANG Shu-ling

doi:10.13422/j.cnki.syfjx.20180804

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Effect of Extraction and Processing on Chemical Constituents in Bupleuri Radix by HPLC-ELSD Fingerprint

更新时间：2024-01-04

- Effect of Extraction and Processing on Chemical Constituents in Bupleuri Radix by HPLC-ELSD Fingerprint
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 7, Pages: 13-17(2018)
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- DOI：10.13422/j.cnki.syfjx.20180804
  CLC： R284.1
- Published：2018
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CONG Meng-yu, GONG Yan-rong, LIANG Sha-bi, et al. Effect of Extraction and Processing on Chemical Constituents in Bupleuri Radix by HPLC-ELSD Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 13-17.
DOI：

CONG Meng-yu, GONG Yan-rong, LIANG Sha-bi, et al. Effect of Extraction and Processing on Chemical Constituents in Bupleuri Radix by HPLC-ELSD Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 13-17. DOI： 10.13422/j.cnki.syfjx.20180804.

摘要

目的：建立柴胡HPLC-ELSD指纹图谱，分析柴胡主要成分在提取和炮制过程中的变化规律。方法：色谱条件为Phenomenex Kinetex® C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈（A）-水（B）梯度洗脱（0~60 min，25%~58% A），柱温30℃，流速1.0 mL ·min-1，进样量10 μL，检测器为蒸发光检测器，氮气流速3.0 mL ·min-1；以此色谱条件分别对4种样品（柴胡水提液、柴胡醇提液、醋柴胡水提液、醋柴胡醇提液）进行指纹图谱研究，并测定柴胡皂苷a，d，b1，b2的含量。结果：得到4种柴胡样品指纹图谱，共指认23个色谱峰，各样品的指纹图谱共有模式图不尽相同，成分色谱峰存在较大差异。柴胡经水提加热回流或醋炙后会使原生皂苷向次生皂苷转化，其中醋柴胡水提液中的原生皂苷含量最少，次生皂苷含量最多，柴胡醇提液中次生皂苷含量最少。结论：所建立的HPLC-ELSD指纹图谱可作为柴胡化学成分分析测定的方法；柴胡经水提或醋炙后化学成分变化较大，含柴胡制剂的临床使用及质量评价应充分考虑到柴胡皂苷类成分的转变情况。

Abstract

Objective: To establish the HPLC-ELSD fingerprint of Bupleuri Radix and analyze the change rule of the main components from Bupleuri Radix in extraction and processing. Method: Phenomenex Kinetex® C18 column (4.6 mm×250 mm

5 μm) was employed with the mobile phase of acetonitrile-water for gradient elution.The flow rate was 1.0 mL · min-1

column temperature was 30℃ and the injection amount was 10 μL.Evaporative light scattering detector was adopted with the flow rate of nitrogen was 3.0 mL · min-1.These chromatographic conditions were used to study on fingerprints of four kinds of samples and determine the contents of saikosaponin a

b2. Result: A total of 23 chromatographic peaks were identified in the fingerprints of four samples of Bupleuri Radix.The common patterns of fingerprints were different from each other and there was a big difference between chromatographic peaks.After high temperature water extraction or processing with vinegar

the primary saponin of Bupleuri Radix was transformed into secondary saponin.Among them

the primary saponin content in the water extract of Bupleuri Radix processed with vinegar was the least

the content of secondary saponin was the most; and the content of secondary saponin was the least in methanol extract of Bupleuri Radix. Conclusion: The established HPLC-ELSD fingerprint can be used as a chemical component analysis method for Bupleuri Radix.The chemical compositions of Bupleuri Radix are greatly changed after water extraction or processing with vinegar.The clinical application and quality evaluation of preparations containing Bupleuri Radix should take into full account of change of saikosaponins.

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