Effect of Mayuan Tongbian Zhitong Decoction on Intestinal Propulsive Function in Rats with Slow Transit Constipation

XIE Chang-ying; WU Cheng-cheng; XIAO Hui-rong

doi:10.13422/j.cnki.syfjx.20180823

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Effect of Mayuan Tongbian Zhitong Decoction on Intestinal Propulsive Function in Rats with Slow Transit Constipation

更新时间：2024-01-04

- Effect of Mayuan Tongbian Zhitong Decoction on Intestinal Propulsive Function in Rats with Slow Transit Constipation
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 7, Pages: 154-158(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.20180823
  CLC： R285.5
- Published：2018
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XIE Chang-ying, WU Cheng-cheng, XIAO Hui-rong. Effect of Mayuan Tongbian Zhitong Decoction on Intestinal Propulsive Function in Rats with Slow Transit Constipation[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 154-158.
DOI：

XIE Chang-ying, WU Cheng-cheng, XIAO Hui-rong. Effect of Mayuan Tongbian Zhitong Decoction on Intestinal Propulsive Function in Rats with Slow Transit Constipation[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 154-158. DOI： 10.13422/j.cnki.syfjx.20180823.

摘要

目的：探究麻元通便止痛汤对慢传输型便秘（STC）大鼠肠道功能、排便量、肠神经递质及水通道蛋白（AQP）的影响，并探讨其可能的作用机制。方法：雄性SD大鼠60只，随机分为正常组、模型组、麻元通便止痛汤低、中、高剂量组（6，12，18 mg ·kg-1）及莫沙必利组；模型组、麻元通便止痛汤组及莫沙必利组采用复方地芬诺酯混悬液10 mg ·kg-1 ·d-1灌胃，连续给药14 d，建立STC模型；模型建立后，给药组分别给予相应药物，正常组及模型组给予等体积0.9% NaCl溶液灌胃，连续给药14 d。检测各组大鼠造模前、造模期及治疗期粪便数量及含水量；计算各组大鼠碳末推进率；酶联免疫吸附法（ELISA）检测各组大鼠结肠组织一氧化氮（NO）及一氧化氮合酶（NOS）含量；蛋白免疫印迹法（Western blot）检测各组大鼠结肠组织水通道蛋白1，3，4，8（AQP1，3，4，8）表达。结果：与正常组比较，模型组大鼠碳末推进率、排便量及粪便含水量降低（P<0.05），与模型组比较，麻元通便止痛汤组及莫沙必利组大鼠碳末推进率、排便量及粪便含水量升高，且呈剂量依赖型（P<0.05）；与正常组比较，模型组大鼠结肠NO，NOS含量升高（P<0.05），与模型组比较，麻元通便止痛汤组及莫沙必利组结肠NO，NOS含量降低，且呈剂量依赖型（P<0.05）；与正常组比较，模型组大鼠结肠AQP1，3，4，8表达升高（P<0.05），与模型组比较，麻元通便止痛汤组及莫沙必利组结肠AQP1，3，4，8表达降低，且呈剂量依赖型（P<0.05）。结论：麻元通便止痛汤可改善STC大鼠肠道功能、排便数量及粪便含水量，其机制可能与减少结肠NO，NOS含量及AQP1，3，4，8表达有关。

Abstract

Objective: To investigate the effect of Mayuan Tongbian Zhitong decoction on intestinal function

defecation

intestinal neurotransmitter and aquaporin (AQP) in rats with slow transit constipation (STC)

and its potential mechanism of action. Method: Sixty male Sprague-Dawley rats were randomly divided into control group

model group

Mayuan Tongbian Zhitong decoction group (6

18 mg · kg-1)

and mosapride group. The model group

Maoyuan Tongbian Zhitong decoction group and Mosapride group were orally given diphenoxylate suspension 10 mg · kg-1 · d-1 for successively 14 d

so as to establish the STC model. After the model was established

the drug groups were respectively given the corresponding drugs

while control group and model group were given the equal volume of 0.9% NaCl solution for successively 14 days. The amount of feces and water content in each group before and during modeling and during treatment were measured. The carbon advancing rate of rats in each group was calculated; the contents of nitric oxide (NO) and nitric oxide synthase (NOS) in the colon tissues of rats in each group were detected by enzyme-linked immunosorbent assay (ELISA). Western blot was used to detect the expressions of Aquaporin 1

4 and 8 (AQP1

4 and 8) in the colon tissues of rats in each group. Result: Compared with the control group

the carbon advancing rate

defecation and fecal water content in model group was decreased (P<0.05); compared with model group

Mayuan Tongtong Zhitong decoction group and mosapride group's carbon acceleration rate and defecation and fecal water content were increased in a dose-dependent manner (P<0.05); compared with the control group

the content of NO and NOS in colon in the model group were increased (P<0.05); compared with model group

the content of NO and NOS in colon of Maoyuan Tongbian Zhitong decoction group and mosapride group were decreased in a dose-dependent manner (P<0.05). Compared with the control group

the expressions of AQP1

4 and 8 in the model group were significantly increased (P<0.05). Compared with the model group

the expressions of AQP1

4 and 8 in Mayuan Zhitong Zhitong decoction group and Mosapride group were decreased in a dose-dependent manner (P<0.05). Conclusion: Mayuan Tongbian Zhitong decoction can improve intestinal function

defecation and fecal water content in STC rats. The mechanism may be related to the decrease of the content of NO and NOS in colon and the expressions of AQP1

4 and 8.

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