Effect of Combined Application of Rhei Radix et Rhizoma-Scutellariae Radix on Prevalence of Epilepsy of Brain Injury

SHI Suo-qin; YAN Yao-yao; MA Yao; WANG Fu-shun

doi:10.13422/j.cnki.syfjx.20180835

您当前的位置：

首页 >

文章列表页 >

Effect of Combined Application of Rhei Radix et Rhizoma-Scutellariae Radix on Prevalence of Epilepsy of Brain Injury

更新时间：2024-01-04

- Effect of Combined Application of Rhei Radix et Rhizoma-Scutellariae Radix on Prevalence of Epilepsy of Brain Injury
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 10, Pages: 168-174(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20180835
  CLC： R285.5
- Published：2018
- 稿件说明：
移动端阅览
SHI Suo-qin, YAN Yao-yao, MA Yao, et al. Effect of Combined Application of Rhei Radix et Rhizoma-Scutellariae Radix on Prevalence of Epilepsy of Brain Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(10): 168-174.
DOI：

SHI Suo-qin, YAN Yao-yao, MA Yao, et al. Effect of Combined Application of Rhei Radix et Rhizoma-Scutellariae Radix on Prevalence of Epilepsy of Brain Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(10): 168-174. DOI： 10.13422/j.cnki.syfjx.20180835.

摘要

目的：研究大黄-黄芩配伍应用对小鼠脑损伤早期癫痫易感性的影响，探讨该药对抗脑损伤后癫痫的机制。方法：将SPF级C57BL/6J雄性小鼠随机分为空白组，模型组，大黄-黄芩药对低（1 g·kg-1），高剂量组（4 g·kg-1）及阳性组（2.6 g·kg-1丙戊酸钠）。除空白组外，其余各组采用自由落体法建立小鼠封闭性颅脑损伤模型，于脑损伤后给予相应药物灌胃治疗，连续7 d。于末次给药后给予戊四氮（pentylenetetrazole，PTZ）亚惊厥剂量刺激，通过行为学观察各组小鼠的癫痫发作潜伏期及平均发作强度，脑电图（electroencephalogram，EEG）分析小鼠的脑部放电图谱，统计0~20 Hz的放电总和；免疫组化法（immunohistochemistry，IHC）计数海马CA1，CA3区胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）的阳性细胞数；蛋白免疫印迹法（Western blot）检测神经元Na+-K+-2Cl-共转运体-1（NKCC1）的蛋白表达水平。结果：与空白组比较，模型组的小鼠癫痫发作潜伏期明显缩短，脑部异常高频放电明显增多，海马CA1，CA3区的GFAP阳性细胞计数显著增加（P<0.05），且神经元NKCC1的表达也较高（P<0.05）；与模型组比较，药对低、高剂量均可延长癫痫发作的潜伏期，减少脑部异常放电，降低海马区GFAP阳性细胞数以及神经元NKCC1蛋白的表达，且药对高剂量组与模型组比较具有显著性差异（P<0.05）。结论：大黄-黄芩配伍应用可降低脑损伤早期癫痫的易感性，作用机制可能与星形胶质细胞的调控作用有关。

Abstract

Objective: To study the effect of the combined application of Rhei Radix et Rhizoma-Scutellariae Radix on the susceptibility of early epilepsy of traumatic brain injury in mice

in order to explore the mechanism of epilepsy after brain injury. Method: SPF-grade C57BL/6J male mice were randomly divided into 5 groups

namely blank control group

model group

low-dose Rhei Radix et Rhizoma-Scutellariae Radix treatment group (1 g·kg-1)

high-dose group (4 g·kg-1)

positive control group (2.6 g·kg-1 sodium valproate). A mouse model of closed brain injury was established by the weight-falling method in all test groups

except for the blank control group. The mice were given the corresponding drugs by gavage for successively 7 days after mice brain injury. Subacute dose of pentylenetetrazole (PTZ) was given to the mice after the last administration. The latency and mean seizure intensity of epileptic seizures in mice were measured through the behavioral observation. EEG changes of mice were analyzed by counting the sum of 0-20 Hz discharges. Positive cells of glial fibrillary acidic protein (GFAP) in hippocampal CA1 and CA3 regions were counted by immunohistochemistry. Protein expression of Na+-K+-2Cl- co-transporter (NKCC1) was detected by Western blot. Result: Compared with the control group

the latency of epilepsy seizure was significantly shortened in the model group

abnormal high-frequency discharge in brain was significantly enhanced

and the number of GFAP positive cells in the hippocampal CA1 and CA3 regions was significantly increased (P<0.05)

and the expression level of NKCC1 in neurons was remarkably increased (P<0.05). Compared with the model group

the low and high-dose Rhei Radix et Rhizoma-Scutellariae Radix could prolong the latency of epileptic seizures

reduce the abnormal discharge of brain

decrease the number of GFAP positive cells and the expression of NKCC1 protein in the hippocampus (P<0.05)

with significant difference between the high-dose group and the model group (P<0.05). Conclusion: The combined application of Rhei Radix et Rhizoma-Scutellariae Radix could reduce the susceptibility of early epilepsy after brain injury

and the mechanism may be related to the regulation of astrocytes.

关键词

Keywords

references

Views

198

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Yiqi Huoxue Tongluo Prescription Inhibits Activation of Astrocytes to Relieve Diabetic Neuropathic Pain in Rats via ERK Signaling Pathway

Mechanism of Dihuang Yinzi in Improving Energy Metabolism Disorder and Autophagy Injury of Astrocytes in Brain of AD Mice

Mechanism of Dihuang Yinzi in Improving Astrocyte Injury and Glycolysis in AD Mice via PI3K/Akt Pathway

Mechanism of Dihuang Yinzi in Improving Astrocyte Injury and Regulating Synaptic Structure and Function in AD Mice

Effect of Zuogui Jiangtang Tongmai Prescription on Astrocyte Injury by AGEs Combined with Oxygen-glucose Deprivation Based on AMPK/mTOR/ULK1 Pathway Related to Autophagy

Related Author

JIANG Aijuan

WANG Yuqing

LI Jiajie

WANG Lujie

CHEN Qian

CHENG Kai

YUAN Huilun

MA Tao

Related Institution

School of Integrated Traditional Chinese and Western Medicine， Anhui University of Chinese Medicine

Institute of Integrated Traditional Chinese and Western Medicine， Anhui Academy of Chinese Medicine

Dongfang Hospital, Beijing University of Chinese Medicine

School of Traditional Chinese Medicine,Beijing University of Chinese Medicine

Japan Friendship Hospital

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰