Objective: To establish a systematic quality control method for different parts of Lagerstroemia indica

including qualitative and quantitative analysis by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC)

in order to determine alcohol-soluble extractives

ash and loss on drying

respectively. Method: TLC was applied

and the development solvent system was a mixture of acetone

petroleum ether formic acid (3:5:0.2)

and the examination was under UV light at a wavelength of 365

254 nm and daylight. HPLC was employed to determinate ellagic acid in different parts of L. indica

in which chromatographic separation was performed on the column Ultimate® AQ-C18 (4.6 mm×250 mm

5 μm) with the temperature at 40℃. Acetonitrile -0.2% and aqueous phosphoric acid (15:85) were taken as the mobile phase for isocratic elution

the flow rate was 1.0 mL · min-1

and the detection wavelength was 254 nm. Alcohol-soluble extractives

total ash and loss on drying were measured based on Chinese Pharmacopoeia 2015 version. Result: The spots in TLC chromatograms of different parts of L. indicae from different batches and regions were clear

and the Rf value was appropriate. There was a good linear relationship (r=0.999 8) of ellagic acid at the concentration range between 0.276 8-55.36 mg · L-1

and the average recovery rates of flos

folium and cortex of L. indicae were 99.64%

99.73% and 99.40%

respectively. There were differences in ellagic acid concentration in various batches of samples

in which flos was higher than folium and cortex. The average contents of alcohol-soluble extractives were 18.61%

18.67% and 3.78%

the average ash contents were 6.50%

7.47% and 5.62%

and the losses on drying content were 10.51%

11.41% and 14.22% in flos

folium and cortex of L. indicae

respectively. Conclusion: The method was simple

accurate and reliable

and can provide the basis for the more effective quality control of different parts L. indica.