Effect of Alismatis Rhizoma Polysaccharide on Glucose-lipid Metabolism in Diabetic Rats

QIAN Zeng-kun; CUI Fan; LING Yun-xi; ZHU Wen-juan; LI Xiao-qin; MAO Zheng; LI Min-ting

doi:10.13422/j.cnki.syfjx.20180938

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Effect of Alismatis Rhizoma Polysaccharide on Glucose-lipid Metabolism in Diabetic Rats

更新时间：2024-01-04

- Effect of Alismatis Rhizoma Polysaccharide on Glucose-lipid Metabolism in Diabetic Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 11, Pages: 117-125(2018)
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- DOI：10.13422/j.cnki.syfjx.20180938
  CLC： R285.5
- Published：2018
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QIAN Zeng-kun, CUI Fan, LING Yun-xi, et al. Effect of Alismatis Rhizoma Polysaccharide on Glucose-lipid Metabolism in Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(11): 117-125.
DOI：

QIAN Zeng-kun, CUI Fan, LING Yun-xi, et al. Effect of Alismatis Rhizoma Polysaccharide on Glucose-lipid Metabolism in Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(11): 117-125. DOI： 10.13422/j.cnki.syfjx.20180938.

摘要

目的：研究泽泻多糖提取物对糖尿病大鼠肝脏糖脂代谢及肝脏脂联素受体α（Adipo R2），过氧化物酶体增殖物激活受体（PPARα） mRNA和蛋白表达的影响，并探讨其可能的作用机制。方法：采用腹腔注射链脲佐菌素（streptozotocin，STZ）建立糖尿病大鼠模型，并随机分组为正常组、模型组、盐酸吡格列酮组、泽泻多糖高、中、低（400，200，100 mg·kg-1）剂量组，每组8只，给予相应的药物治疗30 d。实验结束后，比较各组大鼠的体质量，肝重，葡萄糖耐受量（OGTT），空腹血糖值（FBG），空腹胰岛素水平（FINS），糖化血清蛋白（GSP），糖化血红蛋白（HbA1c），血清总胆固醇（TC），甘油三脂（TG），低密度脂蛋白（LDL），高密度脂蛋白（HDL）及肝脏形态组织学等指标；免疫组化法观察大鼠胰腺胰岛素样生长因子（IGF）水平；实时荧光定量PCR（Real-time PCR）法和蛋白免疫印迹法（Western blot）分别检测肝脏组织中Adipo R2，PPARα mRNA和蛋白表达水平。结果：与模型组比较，泽泻多糖能明显降低糖尿病大鼠的体质量和肝重（P<0.01），并能明显降低大鼠FBG，HbA1c，GSP水平（P<0.05），明显降低TC，TG，LDL水平（P<0.01），升高HDL指标水平（P<0.01）；升高肝脏Adipo R2，PPARα mRNA水平（P<0.05）和蛋白表达水平（P<0.01）。结论：泽泻多糖具有显著调节糖尿病大鼠糖脂代谢的作用，其作用机制与其提高肝脏Adipo R2，PPARα mRNA和蛋白表达水平相关联，并对糖尿病并发症和肝脏组织脂肪积累具有一定的防治作用。

Abstract

Objective: To study the effect of Alismatis Rhizoma polysaccharide (AP) on glucose-lipid metabolism as well as the mRNA and protein expression of Adipo R2 and PPARα in diabetic rats

and discuss the potential mechanism. Method: Diabetic rats model was induced by intraperitoneal injection of streptozotocin (STZ)

and the diabetic rats were randomly divided into six groups

including normal group

model group

Pioglitazone hydrochloride group

AP high

medium and low (400

200

100 mg ·kg-1) dose groups

n=8 in each group. The drug administration was given for 30 days. The body weight

liver weight

oral glucose tolerance test (OGTT)

fasting blood glucose (FBG)

fasting insulin (FINS)

glycosylated serum protein (GSP)

glycosylated hemoglobin (HbA1c)

serum total cholesterol (TC)

triglyceride (TG)

low density lipoprotein (LDL)

high density lipoprotein (HDL) and liver histology index were compared. Immunohistochemical method was used to observe the level of insulin-like growth factors (IGF). The protein expression levels of Adipo R2 and PPARα in liver tissues of different groups were measured with Western blot

and the mRNA expression levels were measured with Real-time PCR. Result: As compared with the model group

AP could significantly reduce the body weight and liver weight of diabetic rats(P<0.01)

as well as the levels of FBG

HbA1c and GSP

improve the indexes of TC

LDL and HDL(P<0.05

P<0.01)

and regulate the mRNA and protein expression levels of Adipo R2 and PPARα in liver tissues(P<0.05

P<0.01). Conclusion: AP can significantly adjust the glucolipid metabolism in diabetic rats

and its potential mechanism is related to the regulation of the expression levels of Adipo R2 and PPARα

with certain prevention and treatment effectfor diabetes complications and fatty accumulation in liver tissues.

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