Objective: To explore the protective effects and mechanism of Shuangshen Tongmai granule (STG) in patients with ischemic reperfusion. Method: SD rats were divided into Sham operation (Sham) group

myocardial ischemia reperfusion injury (MIRI) group

diltiazem hydrochloride (DH) group and STG low dose

middle doseand high dose groups (STG-L

STG-M and STG-H).Intragastric administration was given for two weeks

and then the left anterior descending coronary artery was ligatured for modeling. The left ventricular function and Na+-K+-ATP enzymatic activity were measured;the contents of interleukin-1β (IL-1β)

IL-6 and tumor necrosis factor-α (TNF-α) in plasma were measured by enzyme-linked immunosorbent assay (ELISA). The nuclear transcription factor (NF)-κB

IκBα and Iκκβ protein levels were measured by the techniques of Western blot.Myocardial structure was observed by light microscopy. Result: As compared to MIRI group

the left ventricular function and Na+-K+-ATP enzymatic activitywere significantly improved in STG groups and DH treatment group(P<0.05)

the serum levels of IL-1β

IL-6 and TNF-α were lower (P<0.05).The results of Western blot showed that the protein expressions of NF-κB were lower (P<0.05)

and the protein expressions of IκBα and Iκκβ were higher (P<0.05). Conclusion: STG could alleviatemyocaridial ischemia reperfusion injury inrats by decreasing NF-κB expression protein levels and improving IκBα and Iκκβ expression protein levels.