Isolation and Identification of Endophytic Fungi of Bletillae Rhizoma and Investigation of Antioxidative Activity of Its Exopolysaccharides

DONG Yan-jing; CHENG Fang; XU Xiao-zhen; ZENG Yi; ZHANG Shou-wen

doi:10.13422/j.cnki.syfjx.20181047

您当前的位置：

首页 >

文章列表页 >

Isolation and Identification of Endophytic Fungi of Bletillae Rhizoma and Investigation of Antioxidative Activity of Its Exopolysaccharides

更新时间：2024-01-04

- Isolation and Identification of Endophytic Fungi of Bletillae Rhizoma and Investigation of Antioxidative Activity of Its Exopolysaccharides
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 14, Pages: 24-28(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181047
  CLC： R285
- Published：2018
- 稿件说明：
移动端阅览
DONG Yan-jing, CHENG Fang, XU Xiao-zhen, et al. Isolation and Identification of Endophytic Fungi of Bletillae Rhizoma and Investigation of Antioxidative Activity of Its Exopolysaccharides[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 24-28.
DOI：

DONG Yan-jing, CHENG Fang, XU Xiao-zhen, et al. Isolation and Identification of Endophytic Fungi of Bletillae Rhizoma and Investigation of Antioxidative Activity of Its Exopolysaccharides[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 24-28. DOI： 10.13422/j.cnki.syfjx.20181047.

摘要

目的：缓解白及药用资源压力，分离鉴定白及内生真菌并进行内生真菌胞外多糖活性强弱研究。方法：分离鉴定白及内生真菌，进行液体发酵培养获得胞外多糖，通过建立铁氰化钾法，2

2-联苯基-1-苦基肼基(DPPH)法，水杨酸法，2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)法4种体外模型研究白及内生真菌胞外多糖的抗氧化活性。结果：从白及新鲜根中分离得到10株内生真菌，鉴定合并菌株后得到6株不同的菌株，其中10号菌株胞外多糖(0.1 g·L-1)总还原力最强，其维生素C(VC)当量高达12.01 mg·L-1，其次为6号菌株；清除DPPH自由基活性最强的为1号和6号菌株为16.6%；6号菌株清除羟基自由基活性最强为21.6%，最弱的为10号菌株仅为11.0%；测定其清除ABTS自由基活性时则发现，10号菌株活性最强为13.6%，其次为6号菌株，最弱的为5号仅为1.8%。结论：白及内生真菌胞外多糖具有一定的抗氧化活性，其中6号菌株胞外多糖总还原力，清除DPPH自由基活性，清除羟基自由基活性、清除ABTS自由基均较好，可以加以开发和利用。

Abstract

Objective: To isolate and identify Bletillae Rhizoma fungi and study on the exopolysaccharides activity of endophytic fungi

in order to alleviate the pressure of Bletillae Rhizoma medicinal resources. Method: Bletillae Rhizoma endophytic fungi were isolated and identified

and then exopolysaccharides were obtained through liquid fermentation. The antioxidant activity of exopolysaccharide in Bletillae Rhizoma roots endophytic fungi was studied by establishing 4 kinds of in vitro models

including iron potassium cyanide

2-diphenyl-1-picrylhydrazyl (DPPH)

salicylic acid and 2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. Result: The 10 endophytic fungi were isolated from Bletillae Rhizoma fresh roots

and 6 strains were obtained after identification and combination. The exopolysaccharides of No.10 strain (0.1 g L-1) had the strongest total reducing power

with VC equivalent up to 12.01 mg·L-1

followed by No. 6 strain. No. 1 and No. 6 strains had the strongest DPPH free radical scavenging activities (16.6%). Strain 6 had the strongest hydroxyl radical scavenging activities (21.6%) and No.10 strain had the weakest activity (11.0%) on scavenging hydroxyl radical. In the detection of ABTS free radical scavenging activity

it was found that No. 10 strain had the strongest activity (13.6%)

followed by No. 6 strain

and the weakest was only 1.8% for No. 5 strain. Conclusion: The exopolysaccharides of Bletillae Rhizoma endophytic fungi has certain antioxidant activity

and the exopolysaccharides of No. 6 strain has better total reducing power

higher scavenging activities of DPPH free radicals

hydroxyl radicals

and ABTS free radicals

so it can be further developed and utilized.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Identification of Chemical Constituents of Bidens pilosa and Analysis of Its Anti-gastric Cancer Cell Proliferation Activity in Vitro

Antioxidant and Anti-tumor Effect and Mechanisms of Andrographis paniculata Polysaccharide on Human Cervical Cancer HeLa Cells

Structural Characteristics and Antioxidant Activity Analysis of Polysaccharides from Pinelliae Rhizoma and Its Processed Products Before and After Hydrolysis （Enzymolysis） by Sugar Spectrum

Research Progress in Extraction Technology， Structural Characteristics and Pharmacological Activities of Hippophae rhamnoides Polysaccharides

Role of Flavonoids in Traditional Chinese Medicine to Improve Cerebral Ischaemia-reperfusion Injury and Its Mechanism： A Review

Related Author

HAN Yu

LIU Chang

LIU Jiao

ZHANG Tao

ZOU Zhongmei

HUANG Lijin

LI Zilin

YANG Ziyan

Related Institution

Henan University of Chinese Medicine

Shenyang Pharmaceutical University

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences&Peking Union College

Basic Medicine School， Dali University， Dali

Provincial Key Laboratory of Entomology Biopharmaceutical R&D，Dali

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰