Mechanism of Action of Yiyi Fuzi Baijiang San on Ulcerative Colitis Based on Nrf2 Pathway

FANG Jing; CHEN Jiang; PENG Jun-wei; LI Hong-lin; JIANG Cheng-fan

doi:10.13422/j.cnki.syfjx.20181052

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Mechanism of Action of Yiyi Fuzi Baijiang San on Ulcerative Colitis Based on Nrf2 Pathway

更新时间：2024-01-04

- Mechanism of Action of Yiyi Fuzi Baijiang San on Ulcerative Colitis Based on Nrf2 Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 13, Pages: 85-92(2018)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.20181052
  CLC： R285.5
- Published：2018
- 稿件说明：
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FANG Jing, CHEN Jiang, PENG Jun-wei, et al. Mechanism of Action of Yiyi Fuzi Baijiang San on Ulcerative Colitis Based on Nrf2 Pathway[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(13): 85-92.
DOI：

FANG Jing, CHEN Jiang, PENG Jun-wei, et al. Mechanism of Action of Yiyi Fuzi Baijiang San on Ulcerative Colitis Based on Nrf2 Pathway[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(13): 85-92. DOI： 10.13422/j.cnki.syfjx.20181052.

摘要

目的：探讨薏苡附子败酱散治疗溃疡性结肠炎在抗氧化应激信号通路方面的作用机制研究。方法：60只雄性C57BL/6J小鼠随机均分为正常组、模型组、美沙拉秦组、薏苡附子败酱散低、中、高剂量组。除正常组以外，其余各组小鼠均制备成急性期溃疡性结肠炎模型。造模过程中的第3天，薏苡附子败酱散低、中、高剂量组分别给予5.24，10.48，20.96 g·kg-1的薏苡附子败酱散药液，美沙拉秦药组给予0.82 g·kg-1的美沙拉秦药液；正常组、模型组灌胃给予同体积双蒸水，7 d为1个疗程。实验过程中每日观察小鼠的一般情况，包括体质量变化、大便性状以及粪便隐血等等，绘制每组小鼠的疾病活动指数（DAI）评分表格。实验的第10天处死小鼠，取结肠组织做苏木精-伊红（HE）染色、组织病理（HI）评分，测量肠组织中总超氧化物歧化酶（T-SOD），丙二醛（MDA）的含量，免疫组化法检测结肠组织中的核转录因子E-2-相关因子（Nrf2）和血红素加氧-1（HO-1）蛋白表达，以及用逆转录聚合酶链反应（RT-PCR）技术检测结肠组织中Nrf2 mRNA表达变化。结果：治疗后药物组小鼠与模型组相比，DAI评分明显降低（P<0.05），HE染色肠组织结构尚存，HI评分明显降低（P<0.05）；T-SOD在模型组中的含量明显降低（P<0.05），在药物组中的含量明显升高（P<0.05）；MDA在模型组中的含量明显增高（P<0.05），在药物组中的含量降低（P<0.05）。免疫组化结果显示，Nrf2的表达药物组与模型组相比明显升高（P<0.05）；HO-1的表达与模型组相比，薏苡附子败酱散低剂量组差异不大，薏苡附子败酱散高剂量组表达明显增高（P<0.05）。RT-PCR检测Nrf2 mRNA的表达结果发现，与模型组相比，薏苡附子败酱散高剂量组高表达（P<0.05）。结论：薏苡附子败酱散能够上调Nrf2及其下游抗氧化蛋白HO-1的表达，增加Nrf2 mRNA表达，对溃疡性结肠炎有治疗作用。

Abstract

Objective: To study the active mechanism of Yiyi Fuzi Baijiang San in treating ulcerative colitis in antioxidant stress signal pathway. Method: Sixty male C57BL/6J mice were randomly divided into six groups

normal group

model group

Mesalazine group

and low

medium and high-dose Yiyi Fuzi Baijiang San groups. Except for the normal group

the other groups of mice were included in the acute ulcerative colitis model. At the 3rd day of modeling

low

medium and high-dose Yiyi Fuzi Baijiang San groups were given liquid Chinese medicine with doses of 5.24

10.48

20.96 g·kg-1. The Mesalazine drug group was treated with 0.82 g·kg-1 of mesalazine solution. Normal group and model group were intragastrically given the same volume of double distilled water. A course of treatment was 7 days. Weight changes

stool traits and fecal occult blood of the mice were observed

and the disease activity index (DAI) score curve was drawn for each group of mice. The mice were put to death on the 10th day of the experiment; colon tissues were taken for hematoxylin-eosin(HE) staining and HI scoring; the contents of total superoxide dismutase (T-SOD) and malondialdehyde (MDA) in intestinal tissues were measured; and the expression of Nrf2 and hemeoxygenase (HO)-1 protein in colon tissues were detected by immunohistochemical method. The changes in Nrf2 mRNA expression in tissues were detected by RT-PCR. Result: Compared with the model group

the DAI score of the drug-treated mice decreased significantly (P<0.05)

the histological structure of the intestinal mucosa was survived

and the HI score decreased significantly (P<0.05). The content of T-SOD in the model group was significantly lower (P<0.05)

and significantly higher in the drug groups (P<0.05); the content of MDA in the model group was significantly higher (P<0.05) and decreased in the drug group (P<0.05). Immunohistochemistry showed that the expression of Nrf2 was significantly increased in the drug-treated groups compared with the model group (P<0.05). Compared with the model group

the expression of HO-1 in the low-dose group was not significantly different. High-dose group was significantly higher (P<0.05). RT-PCR detection of Nrf2 mRNA expression found that compared with the model group

high-dose group showed a high expression (P<0.05). Conclusion: Yiyi Fuzi Baijiang San can up-regulate the expression of Nrf2.Its downstream antioxidant protein HO-1 can increase the Nrf2 mRNA expression and have a therapeutic effect on ulcerative colitis.

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