Effect of Shenling Baizhu San on Expressions of IL-13, IL-23 and COX-2, CREB in Ulcerative Colitis Rats with Spleen Deficiency and Dampness

DING Ling-hui; JIA Yu-xin; CHENG Ying-xia; MA Wen; DUAN Yong-qiang; MING Hai-xia; WAN Sheng-fang; CHENG Xiao-li

doi:10.13422/j.cnki.syfjx.20181058

您当前的位置：

首页 >

文章列表页 >

Effect of Shenling Baizhu San on Expressions of IL-13, IL-23 and COX-2, CREB in Ulcerative Colitis Rats with Spleen Deficiency and Dampness

更新时间：2024-01-04

- Effect of Shenling Baizhu San on Expressions of IL-13, IL-23 and COX-2, CREB in Ulcerative Colitis Rats with Spleen Deficiency and Dampness
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 11, Pages: 67-72(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181058
  CLC： R285.5
- Published：2018
- 稿件说明：
移动端阅览
DING Ling-hui, JIA Yu-xin, CHENG Ying-xia, et al. Effect of Shenling Baizhu San on Expressions of IL-13, IL-23 and COX-2, CREB in Ulcerative Colitis Rats with Spleen Deficiency and Dampness[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(11): 67-72.
DOI：

DING Ling-hui, JIA Yu-xin, CHENG Ying-xia, et al. Effect of Shenling Baizhu San on Expressions of IL-13, IL-23 and COX-2, CREB in Ulcerative Colitis Rats with Spleen Deficiency and Dampness[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(11): 67-72. DOI： 10.13422/j.cnki.syfjx.20181058.

摘要

目的：观察参苓白术散对脾虚湿困型溃疡型结肠炎大鼠结肠组织白细胞介素（IL）-13，IL-23及环氧合酶-2（COX-2），cAMP反应元件结合蛋白（CREB）表达的影响，探究其对溃疡性结肠炎作用机制。方法：将48只SPF级Wistar大鼠随机分为6组，分别是空白组、模型组、参苓白术散高、中、低剂量组和美沙拉秦组，每组8只，雌雄各半。除空白组，其余各组采用病证结合法复制脾虚湿困型溃疡性结肠炎大鼠模型，即饮食、环境干预等措施联合2，4-二硝基苯磺酸灌肠。造模成功后，参苓白术散高、中、低剂量组分别按照24，12，6 g·kg-1·d-1，美沙拉秦组0.2 g·kg-1·d-1进行灌胃。连续灌胃21 d后采用酶联免疫吸附法（ELISA）检测大鼠结肠组织IL-13，IL-23含量；实时荧光定量聚合酶链式反应法（Real-time PCR）检测结肠组织IL-13，IL-23，COX-2，CREB mRNA表达；蛋白免疫印迹法（Western blot）检测结肠组织COX-2，CREB蛋白表达量。结果：与空白组比较，模型组大鼠结肠IL-13含量和IL-13 mRNA表达水平明显降低（P<0.05），IL-23含量和IL-23 mRNA升高（P<0.05），COX-2，CREB mRNA及蛋白相对表达量升高（P<0.05）。与模型组比较，参苓白术散（高、中、低剂量）组大鼠结肠IL-13含量和IL-13 mRNA表达均升高，尤以参苓白术散高剂量组最显著（P<0.05），IL-23含量和IL-23 mRNA降低（P<0.05），COX-2，CREB mRNA表达及蛋白相对表达量降低（P<0.05）。结论：参苓白术散可能调节脾虚湿困型溃疡性结肠炎大鼠结肠IL-13，IL-23及COX-2，CREB的表达，减少结肠的炎症反应。

Abstract

Objective: To observe the effect of Shenling Baizhu San on the expressions of interleukin (IL)-13

IL-23 and cyclooxygenase-2 (COX-2)

CAMP response element binding protein (CREB) in ulcerative colitis rats with spleen deficiency and dampness

in order to explore its mechanism. Method: Totally 48 SPF Wistar rats were randomly divided into 6 groups: blank group

model group

high

medium and low-dose Shenling Baizhu San groups

and mesalazine group

with 8 rats in each group

half male and half female. Except for blank group

the rats in the other groups were included into the saphenous ulcerative colitis model by the combination of disease and syndrome

including diet

environmental intervention and 2

4-dinitrobenzene sulfonic acid enema. After successful modeling

Shenling Baizhu San group was respectively treated with the drug at high dose

middle dose and low dose (24

6 g·kg-1·d-1)

and mesalazine group was treated with the drug (0.2 g·kg-1·d-1) for gavage. After 21 days of treatment

the levels of IL-13 and IL-23 in colonic tissues were detected by enzyme-linked immunosorbent assay (ELISA); mRNA expressions of IL-13

IL-23

COX-2

CREB in colonic tissues were detected by Real-time PCR; the protein expressions of COX-2

CREB in colonic tissues were detected by Western blot. Result: Compared with the blank group

the model group rats' IL-13 content and IL-13 mRNA expression in colon were significantly decreased (P<0.05)

IL-23 content and IL-23 mRNA expression increased (P<0.05)

COX-2

CREB mRNA expression and relative protein expression increased (P<0.05). Compared with the model group

IL-13 and IL-13 mRNA expressions in the colonic group were significantly higher than the rats treated with Shenling Baizhu San (high

medium and low doses)

especially in the high-dose group (P<0.05)

IL-23 content and IL-23 mRNA expression decreased (P<0.05)

and COX-2

CREB mRNA expressions and relative protein expression decreased (P<0.05). Conclusion: Shenling Baizhu San can strengthen spleen Qi and excrete moisture

which may regulate the expressions of IL-13

IL-23 and COX-2

CREB in the colon of rats with spleen deficiency and dampness

so as to treat colonic ulcer.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Shenling Baizhu San on Expressions of NLPR3, NLPR6 Protein and Related Inflammatory Factors in Mice with Ulcerative Colitis

Meta-analysis on Effectiveness and Safety of Modified Shenling Baizhu San and Western Medicines in Treating Ulcerative Colitis

Nano Oral Colon Targeted Drug Delivery System in Treatment of Ulcerative Colitis： A Review

Comparison of Pharmacokinetics and Tissue Distribution of Free Anthraquinones and Gallic Acid in Mice with Ulcerative Colitis Before and After Processing of Rhei Radix et Rhizoma Carbonisata

Mechanisms of Zhuyuwan in Treating both Intrahepatic Cholestasis and Ulcerative Colitis Based on Homotherapy for Heteropathy

Related Author

Jia-jun ZHANG

Jia-xi SHI

Juan HUANG

Xu-guang SHI

Cui-ying LIU

Qi WANG

WENG Xiang-tao

HU Yue

Related Institution

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine

First College of Clinical Medicine, Guangzhou University of Chinese Medicine

Foshan Chinese Hospital

Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine

Jiangxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰