LIU Peng-peng, CHEN Jiang-ning, JIA Tian-zhu. Analysis of Diversity of Endophytic Fungi in Astragali Radix by Illumina MiSeq High-throughput Sequencing Technology[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(12): 34-38.
DOI:
LIU Peng-peng, CHEN Jiang-ning, JIA Tian-zhu. Analysis of Diversity of Endophytic Fungi in Astragali Radix by Illumina MiSeq High-throughput Sequencing Technology[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(12): 34-38. DOI: 10.13422/j.cnki.syfjx.20181105.
Analysis of Diversity of Endophytic Fungi in Astragali Radix by Illumina MiSeq High-throughput Sequencing Technology
Objective: This study aimed to analysis the diversity of endophytic fungal communities in Astragali Radix
and provide a reference for study on the directional processing technology of active ingredients in this herb. Method: The 18S rDNA NS1-Fung region of endophytic fungal communities in Astragali Radix was sequenced by Illumina MiSeq high-throughput sequencing technology
abundance of species and other bioinformation were analyzed. Result: The numbers of effective sequences and operational taxonomic units for samples were 29 677 and 188
respectively.The number of sequencing was close to saturation
and the sequencing data volume was reasonable.The main endophytic fungi of Astragali Radix belonged to 5 genera
such as Hemileia
Gibberella
Ascobolus
Pseudogymnoascus and Aspergillus. Conclusion: By and large
the diversity of endophytic fungi in Astragali Radix is low.Hemileia and Gibberella are the dominant endophytic fungi in Astragali Radix.
Diversity and Anti-Dermatophyte Activities of Endophytic Fungi from Datura metel
Isolation and Identification of Secondary Metabolites from Endophytic sp. in Astragali Radix
Effect of Different Fermentation Conditions on Fungal Community and Chemical Composition of Aurantii Fructus
Investigation of Component Difference of Astragali Radix Before and After Rice Stir-frying by UPLC-Q-TOF-MS/MS Combined with Chemometrics
Clinical Efficacy of Modified Huangqi Chifengtang in Treatment of IgA Nephropathy Patients and Exploration of Dose-effect Relationship of Astragali Radix
Related Author
Mei-ru FENG
Wen-guang SHI
Jia-qi LI
Dan-ting CHEN
Ying-hao FU
Zu-jun DENG
ZHANG Hong-chi
LIU Rui
Related Institution
Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances,School of Life Sciences and Biopharmaceutics,Guangdong Pharmaceutical University
Jiangxi Guxiang Jinyun Comprehensive Health Industry Co. Ltd.
Key Laboratory of Depression Animal Model Based on Traditional Chinese Medicine(TCM)Syndrome of Jiangxi Province Administration of TCM,Key Research Office for Evaluation of TCM Efficacy(Prevention and Treatment of Mental Disorders and Brain Diseases) of Jiangxi Province Administration of TCM, Jiangxi University of Chinese Medicine