Objective: To establishultra high performance liquid chromatography (UPLC) method

analyze the contents of flavonoids and phenols ingredients in the different parts of Schisandra chinensis(SC)

and evaluate their in vitro antioxidant capacities. Method: Analysis was performed on an ACQUITY UPLC BEH C18(2.1 mm×5.0 mm

1.7 μm) column with 0.01% phosphoric acid-acetonitrile as the mobile phase for gradient elution. The column temperature was maintained at 30℃. The detection wavelength of flavonoids was set at 360 nm

at the flow rate of 0.35 mL·min-1;and the detection wavelength of phenols was set at 280 nm

at the flow rate of 0.30 mL·min-1. Their antioxidant activities were compared and evaluated by DPPH and ABTS methods. Result: The results showed that the contents of flavonoids and phenols in different parts of SC were significantly different. The content of total flavonoids in leaves was about 3.8 folds and 49.5 folds of that in stems and fruits

respectively. The content of total phenols in leaves was about 4.2 folds and 21.5 folds of that in stems and fruits

respectively. The antioxidant activities of leaves and stems were significantly higher than those in fruits. The scavenging capabilities on DPPH free radicals in leaves and stems were 8.3 folds and 4.3 folds of that in fruits

respectively. The total antioxidant capacities with ABTS method in leaves and stems were 2.9 folds and 1.3 folds of that in fruits

respectively. Conclusion: The contents of total flavonoids and total phenols were positively correlated with their antioxidant activities

demonstrating that the antioxidant activity of SC may be related to the contents of total flavonoids and total phenols. The method was simple

accurate and reproducible

which can be used for the quantification of flavonoids and phenols ingredients of SC

providing the basis for clinical reasonable application of different parts of SC.