Objective: To investigate the effects of Banxia Xiexin Tang medicated serum on the Helicobacter pylori (HP)-infected GES-1 cells through transforming growth factor-β(TGF-β)/Smad signaling pathway to reveal the potential effect and mechanism of this prescription in treatment of heal peptic ulcer. Method: The GES-1 cells were divided into 7 groups

including blank control group

HP-infected model group

healthy human serum group

Rabeprazole serum group

pre-medicated serum group

10% and 20% Banxia Xiexin Tang serum groups. Expect those in blank control group

HP was vaccinated in all the other groups to establish helicobacter pylori model

and then Rabeprazole serum

Banxia Xiexin Tang medicated serum and premedication serum and healthy human serum were adopted for intervention. After 24 hours

cells and their supernatant were collected

and methylthiazolyldiphenyl-tetrazolium bromide(MTT) assay was used to test the cell proliferation; the protein expression levels of Smad2/3

Smad7

transforming growth factor-β1 (TGF-β1) and phosphorylated Smad2/3 (p-Smad2/3)

phosphorylated Smad7 (p-Smad7) were detected by using Western blot. Result: As compared with the blank control group

HP significantly inhibited proliferation of GES-1 cells

decreased protein expression levels of Smad2/3

TGF-β1 and p-Smad2/3 in GES-1 cells

and increased protein expression levels of Smad7 and p-Smad7 (P<0.05

P<0.01). As compared with HP-infected model group

Rabeprazole serum group and 10%

20% Banxia Xiexin Tang serum groups obviously decreased the inhibition on proliferation of GES-1 cells

increased protein expression levels of Smad2/3

TGF-β1 and p-Smad2/3 in GES-1 cells

and significantly decreased Smad7 and p-Smad7 protein expression levels (P<0.05

P<0.01). As compared with pre-medicated serum group

the inhibition on proliferation of GES-1 cells was decreased in Rabeprazole serum group and Banxia Xiexin Tang serum groups; the protein expression levels of Smad2/3

TGF-β1 and p-Smad2/3 in GES-1 cells were increased and the protein expression levels of Smad7 and p-Smad7 were decreased in Rabeprazole serum group and 20% Banxia Xiexin Tang medicated serum group (P<0.05

P<0.01). Conclusion: Banxia Xiexin Tang alleviated HP-induced GES-1 cells injury

promoted GES-1 cells proliferation

and improved the curing quality of ulcers in the treatment of HP-associated peptic ulcer. The mechanism may be related to regulating the TGF-β/Smad signaling pathway.