LAN Rui, Zhang YOng, MA Yun-zhi, et al. Effect of Xiao Xumingtang on Expression of BDNF and GDNF in Acute Cerebral Ischemia Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(15): 149-154.
DOI:
LAN Rui, Zhang YOng, MA Yun-zhi, et al. Effect of Xiao Xumingtang on Expression of BDNF and GDNF in Acute Cerebral Ischemia Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(15): 149-154. DOI: 10.13422/j.cnki.syfjx.20181137.
Effect of Xiao Xumingtang on Expression of BDNF and GDNF in Acute Cerebral Ischemia Reperfusion Injury
Objective: To observe the effects of Xiao Xumingtang (XXMT) on brain derived neurotrophic factor (BDNF)and glial cellderived neurotrophic factor (GDNF)expression levels after acute cerebral ischemia reperfusion injury in rats. Method: A total of 60 male Sprague-Dawley rats were randomly divided into 5 groups:sham operation group (Sham)
model group (IR)
and XXMT low dose
middle dose and high dose groups (15
30
60 g·kg-1·d-1). The Drugs were given from 3 days efore modeling to the end of observation nodes. Cerebral ischemia reperfusion injury models were established by using middle cerebral artery occlusion. Then the pathological changes were estimated by hematoxylin-eosin (HE) staining and Nissl staining at 24 h after reperfusion. BDNF and GDNF expression levels after cerebral ischemia reperfusion injury were quantitatively determined by Western blot and immunohistochemistry. Result: The results indicated that XXMT middle and high dose (30
and up-regulated the expression levels of BDNF and GDNF as compared with IR group(P<0.05). Conclusion: These findings indicated that XXMT treatment may exert the neuroprotective effect on cerebral ischemia by up-regulating expression levels of BDNF and GDNF.
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