Comparison Analysis of Protein, and Macroelements, Trace Elements Composition in Bone and Bone Marrow of Four Types of Xinjiang Characteristic Animals

doi:10.13422/j.cnki.syfjx.20181208

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Comparison Analysis of Protein, and Macroelements, Trace Elements Composition in Bone and Bone Marrow of Four Types of Xinjiang Characteristic Animals

更新时间：2024-01-04

- Comparison Analysis of Protein, and Macroelements, Trace Elements Composition in Bone and Bone Marrow of Four Types of Xinjiang Characteristic Animals
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 12, Pages: 39-46(2018)
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- DOI：10.13422/j.cnki.syfjx.20181208
  CLC： R284.1
- Published：2018
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Comparison Analysis of Protein, and Macroelements, Trace Elements Composition in Bone and Bone Marrow of Four Types of Xinjiang Characteristic Animals[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(12): 39-46.
DOI：

Comparison Analysis of Protein, and Macroelements, Trace Elements Composition in Bone and Bone Marrow of Four Types of Xinjiang Characteristic Animals[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(12): 39-46. DOI： 10.13422/j.cnki.syfjx.20181208.

摘要

目的：测定新疆特色羊、牛、马、骆驼4种动物腿骨骨髓粉末，骨髓油，骨髓蛋白及骨质蛋白中常量微量元素含量及骨髓蛋白相对分子质量，为传统动物药质量标准完善提供参考。方法：用电感耦合等离子发射光谱法（ICP-OES）测定Ca，K，Na，B，P，Ba，Cr，Cu，Fe，Mg，Mn，Ni，Sr，Zn，Si 15种元素含量，并用一位电泳查看骨髓蛋白相对分子质量。结果： 4种骨髓蛋白条带均显示在66 kDa处，各元素线性关系良好，检出限在0.13～3.99 μg·L -1，精密度RSD在0.2%～2.7%，回收率在92.5%～101.4%，大量常量、微量元素均富集在骨质和骨髓蛋白中，其中马骨髓蛋白有益元素最丰富，其中Ca，P质量分数分别高达24.92，10.83 mg·g-1。Na，K，Ca，P，Mg等5种元素含量为骨质蛋白 > 骨髓蛋白 > 骨髓粉末 > 骨髓油；Fe，Cu，Zn，Mn等其他元素含量为骨髓蛋白 > 骨质蛋白 > 骨髓粉末 > 骨髓油。结论：建立的ICP-OES适用于各类动物骨质和骨髓样品中常量微量元素比较分析，灵敏度高。建议以蛋白、多肽为重点对动物药骨髓和骨质进行开发利用，既能够提高其综合利用率，也为相关功能食品、药品的研发提供理论依据。

Abstract

Objective: To determine the contents of macroelements and trace elements in bone marrow powder (BMPD)

bone marrow oil (BMO)

bone marrow protein (BMP) and bone protein (BP) from 4 types of Xinjiang characteristic animals:sheep

bovine

horse

and camel

measure the molecular weight of BMP

and provide a reference for improving the quality standard of traditional animal drugs. Method: Inductively coupled plasma-atomic emission spectrometry(ICP-OES) was used to determine the contents of 15 elements including Ca

Zn and Si;1-DE electrophoresis was used to identify the molecular weight of BMP. Result: Four types of animals BMP bands were all shown at 66 kDa; the trace elements showed good linearity; detection limits range was 0.13-3.99 μg·L -1

with precision RSD of 0.2%-2.7% and recovery rate of 92.5%-101.4%. A large number of constant and trace elements were enriched in BP and BMP

of which horse BMP showed the highest level of beneficial trace elements

with a content of 24.92

10.83 mg·g-1 respectively for Ca and P;the content of Na

Mg was as follows:BP>BMP>BMPD>BMO;the content of Fe

Mn and other elements:BMP>BP>BMPD>BMO. Conclusion: The established ICP-OES method was suitable and highly sensitive for the comparative analysis of trace elements in BM and bone samples from different sources of domestic animals. It was suggested that

protein and peptide should be extracted intensively to improve not only the comprehensive utilization of bone and bone marrow

and also could provide theoretical basis for the research and development of functional foods and medicines.

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