AYIGU·, Tureke, ABDIRYIM·, et al. Effect of Total Flavonoids Extract of on Serum Metabolites in Asthmatic Rat Model[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 76-83.
DOI:
AYIGU·, Tureke, ABDIRYIM·, et al. Effect of Total Flavonoids Extract of on Serum Metabolites in Asthmatic Rat Model[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 76-83. DOI: 10.13422/j.cnki.syfjx.20181305.
Effect of Total Flavonoids Extract of on Serum Metabolites in Asthmatic Rat Model
Objective: To investigate the effect of total flavonoids extract of Adiantum capillus-veneris (TXJF) on serum metabolites of asthmatic rats. Method: Based on the establishment of asthmatic rat model
asthma model rats were given TXJF by intragastric administration
then to observe the percentage of leukocyte in bronchoalveolar lavage fluid(BALF) and pathological changes of lung tissue;levels of immunoglobulin E(IgE)
interleukin-4 (IL-4) and IL-5 in serum of rats were detected.1H-NMR was employed to establish fingerprints of serum metabolites in rats
partial least squares discriminant analysis(PLS-DA) and orthogonal partial least squares discriminant analysis(OPLS-DA) were adopted to analyze the differences in serum metabolites. Result: Compared with asthma model group
total number of leukocytes and inflammatory cells in BALF of TXJF administration group were decreased and the pathological changes of lung tissue were significantly alleviated.The levels of IgE
IL-4 and IL-5 in serum from TXJF high and middle dose groups were significantly lower than those in the asthma model group.The results of PLS-DA showed that the contour differentiation between the asthma model group and the normal group
the TXJF administration group was distinctly separated.Compared with the normal group
the levels of leucine
glycoprotein
glycine
alanine and other metabolites in serum of the asthma model group were decreased;the contents of lactic acid
very low density lipoprotein(VLDL) and acetone were increased(P<0.05).In the TXJF high dose group
the contents of leucine
alanine
glycoprotein
glycine and other compounds were all higher than those of the asthma model group;the contents of acetone
lactic acid
unsaturated lipids
VLDL and other compounds were decreased(P<0.05). Conclusion: TXJF can improve the pathological changes of lung tissue in asthmatic rats
decrease concentrations of IgE
IL-4 and IL-5 in serum and affect the effect of serum metabolites;its mechanism may be related to amino acids metabolism
energy metabolism
fat metabolism and glucose metabolism in asthmatic rat model.
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