Objective: To establish an analysis method for high performance liquid chromatography (HPLC) fingerprint of Opisthoplatia orientalis

quantitatively determine six amino acids in O. orientalis

and provide reference for the quality standards of Opisthoplatia orientalis. Method: Pre-column derivitization method with phenyl isothiocyanate (PITC)-HPLC was used on column Waters XBridge C18(4.6 mm×250 mm

5 μm)

with 0.1 mol·L-1natrium aceticum buffer solution (pH 6.5)-acetonitrie (95:5)(A) and acetonitrie-water (4:1) (B)as mobile phases for gradient elution;the detective wave length was set at 254 nm;the flow rate was 0.8 mL·min-1 and the column temperature was maintained at 40℃. The similarity was analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004A" and the cluster analysis was performed by SPSS 22.0 software. Result: The fingerprint of O. orientalis was established and 21 common peaks were found in the fingerprint

13 of which were identified as amino acid compositions. By considering the similarity analysis

cluster analysis and quantitative analysis of 14 batches of O. orientalis samples

it was found that the similarity analysis and cluster analysis were basically consistent in results

and it was found that the content of arginine may be related to the origin. Conclusion: The established method for HPLC fingerprint of O. orientalis

can comprehensively reflect the amino acid composition of the herbs

providing reference for the identification of medicinal materials and formulation of quality standards for O. orientalis.