SHI Zhao-hua, YE Li-chun, GUAN Xiao-yu, et al. HPLC Fingerprint of Aesculi Semen and Its Application in Identification of Medicinal Materials[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 52-56.
DOI:
SHI Zhao-hua, YE Li-chun, GUAN Xiao-yu, et al. HPLC Fingerprint of Aesculi Semen and Its Application in Identification of Medicinal Materials[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 52-56. DOI: 10.13422/j.cnki.syfjx.20181307.
HPLC Fingerprint of Aesculi Semen and Its Application in Identification of Medicinal Materials
Objective: To establish an analysis method for high performance liquid chromatography (HPLC) fingerprint of Aesculi Semen
detect the chemical constituents in Aesculi Semen from different origins and conduct cluster analysis
providing basis for its identification and quality control. Method: The conditions of separation and detection were achieved on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm
5 μm) with acetonitrile -0.2% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL·min-1; the detection wavelength was 220 nm; the column temperature was 30℃
and the injection volume was 10 μL. The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004A) was adopted for fingerprint analysis and similarity evaluation of the 36 batches of Aesculi Semen samples
and the identification method was discussed in combination with the characteristics of medicinal materials. SPSS 19.0 statistical software was used for cluster analysis. Result: A preferable method for fingerprint determination of Aesculus was established
with a similarity of 0.657-0.994.The medicinal materials were divided into four categories
including A. wilsonii
A. chinensis
A. chinensis var. chekiangensis and its adulterants A. wangii var. wangii. By comparing with control products
two chemical components were identified as escin A and escin B. 36 batches of these samples were also divided into 4 categories in cluster analysis
consistent with the similarity classification results. Conclusion: This study can be used for the determination of Aesculi Semen fingerprint and provides a theoretical basis for its further quality study.
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Related Author
SU Su-jiao
LI Na
Chen Liang
LIN Pei-ling
DING Chun-hua
CHEN Shi-qiang
LIANG Yi-chi
JIANG Yong-mei
Related Institution
College of Pharmacy, Liaoning University of Traditional Chinese Medicine
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