Objective: To discuss the effect of Qingzao Jiufei Tang on the lung cancer cells proliferation related production of glycolysis lactic acid in Lewis-bearing mice

and investigate its possible mechanism. Method: 50 male C57BL/6J mice were randomly divided into model group

chemotherapy group

high

medium and low dose Qingzao Jiufei Tang groups

n=10 in each group. Animal models were induced with the right subaxillary injection of Lewis cells

and Qingzao Jiufei Tang groups started medication two weeks before modeling (11

5.5

and 2.75 g·kg-1·d-1); in chemotherapy group

drug was given by intraperitoneal injection at 50 mg·kg-1·(2 d)-1; same volume of saline was given by intragastric administration after two weeks of drug administratio. After 14 d modeling

the mice were killed and tumors were weighed. The protein expression levels of hypoxia inducible factor (HIF)-1α and cancer-myc(C-myc) were detected by Western blot method

and lactate dehydrogenase-A (LDH-A) activity was detected by enzyme linked immunosorbent assay (ELISA). Result: As compared with model group

the tumor weight

lactic acid content were significantly reduced(P<0.01)

and the protein expression of C-myc in lung cancer cells and LDH-A activity were reduced (P<0.05

P<0.01) in Qingzao Jiufei Tang high dose group

medium dose group

low dose group and chemotherapy group(P<0.01);the protein expression of HIF-1α was significantly reduced in Qinzao Jiufei Tang high and medium dose groups and chemotherapy group (P<0.01). Conclusion: Qingzao Jiufei Tang can inhibit the proliferation of Lewis mice lung cancer cells by inhibiting the expression of HIF-1α protein

down-regulating C-myc protein

reducing the activity of LDH-A and reducing the production of lactic acid in lung cancer cells.