Objective: To investigate the effect of Huanglian Jiedutang on the activation of nuclear transcription factor κB (NF-κB) and the level of inflammatory factors including interleukin-1β (IL-1β)

IL-6 and tumor necrosis factor-α (TNF-α) of HT22 cells induced by β-amyloid protein 25-35 (Aβ25-35). Method: The HT22 cells were cultured and divided into five groups:blank group

model group

donepezil-treated group (0.000 9 g·kg-1) and low and high doses Huanglian Jiedutang-treated groups(2.7

8.1 g·kg-1). The HT22 cells in blank group and model group were treated with blank serum and various treatment groups were treated with corresponding drug-serum and cultured for 1 h

following the incubation with 40 μmol·L-1 Aβ25-35 for 24 h in model group and treatment groups. Then the protein expression level of p-NF-κB p65 was detected by Western blot. Nuclear translocation of NF-κB p65 was determined by immunofluorescence assay. The protein expression and mRNA expression levels of IL-1β

IL-6 and TNF-α were respectively detected by enzyme-linked immunosorbent assay (ELISA) and real time fluorescence quantification polymerase chain reaction (Reat-time PCR). Result: As compared with the blank group

the protein and mRNA expression levels of IL-1 β

IL-6 and TNF-α and the activation level of NF-κB were increased in the model group (P<0.05). As compared with the model group

low and high dose Huanglian Jiedutang and donepezil could decrease the inflammatory factors and mRNA expression levels (P<0.05) and inhibit NF-κB activation. Conclusion: Huanglian Jiedutang may reduce the Aβ25-35-induced HT22 cells inflammatory response and relieve Aβ25-35 neurotoxicity

which may be related to inhibiting the activation of NF-κB.