Comparison of HPLC Fingerprints of Different Processed Products of Farfarae Flos

XUE Fei-fei; WANG Bo; LYU Chen-zi; HE Mei-jing; ZHANG Shuo-sheng

doi:10.13422/j.cnki.syfjx.20181405

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Comparison of HPLC Fingerprints of Different Processed Products of Farfarae Flos

更新时间：2024-01-04

- Comparison of HPLC Fingerprints of Different Processed Products of Farfarae Flos
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 14, Pages: 7-14(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181405
  CLC： R284.1
- Published：2018
- 稿件说明：
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XUE Fei-fei, WANG Bo, LYU Chen-zi, et al. Comparison of HPLC Fingerprints of Different Processed Products of Farfarae Flos[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 7-14.
DOI：

XUE Fei-fei, WANG Bo, LYU Chen-zi, et al. Comparison of HPLC Fingerprints of Different Processed Products of Farfarae Flos[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 7-14. DOI： 10.13422/j.cnki.syfjx.20181405.

摘要

目的：采用HPLC指纹图谱技术，综合运用相似度评价、聚类分析(CA)及主成分分析(PCA)对款冬花炮制前后化学成分变化进行研究。方法：采用HPLC-DAD建立10个产地款冬花不同炮制品的指纹图谱，流动相乙腈-0.03%三氟乙酸水溶液梯度洗脱，检测波长240 nm；运用“中药色谱指纹图谱相似度评价系统”(2004A版)软件，CA及PCA综合探索款冬花不同炮制品的质量及成分变化规律。结果：建立了不同炮制品的HPLC共有模式，款冬花生品、蜜炙品、甘草炙品指纹图谱的相似度分别为0.867~0.991，0.785~0.979，0.785~0.980。CA将不同炮制品均分为4类，与相似度评价结果基本一致，表明不同产地间款冬花炮制品具有一定的相似性和稳定性。PCA筛选出方差累计值达74.230%的2个主成分，并得到与之相关的成分群，不同产地炮制品的综合得分情况表明该成分群在生品、蜜炙品及甘草炙品中稳定存在。结论：指纹图谱技术结合相似度评价，CA，PCA能对款冬花炮制品的质量进行系统评价，可为其他中药炮制品的评价提供参考。

Abstract

Objective: To study on the changes of chemical composition before and after processing of Farfarae Flos by HPLC fingerprint

similarity evaluation

cluster analysis(CA) and principal component analysis(PCA). Method: HPLC-DAD was employed to establish the fingerprints of different processed products of Farfarae Flos from 10 areas.The similarity evaluation

CA and PCA were used to analyze the quality and the changes in composition of different processed products of Farfarae Flos. Result: The common mode of HPLC characteristic chromatographic profile of different processed products was established

the similarities of raw products

honey-fried products and Glycyrrhizae Radix et Rhizoma-fried products were 0.867-0.991

0.785-0.979

0.785-0.980

respectively.Different processed products were divided into four categories by CA

and the results were similar with the similarity evaluation

indicating that the different processed products from different areas had a certain similarity and stability.PCA result screened 2 principle components with accumulative contribution rate of 74.230%

and obtained the related components group

at the same time

the comprehensive score of these processed products from different areas showed that the components group was stable in different processed products of Farfarae Flos. Conclusion: HPLC fingerprint combined with similarity evaluation

PCA can systematically evaluate the quality of processed products of Farfarae Flos

which can provide reference for evaluation of processed products of other Chinese medicines.

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Related Institution

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