Objective: To separate and identify chemical constituents from Cynodon dactylon which lay a foundation for further research on the effective material of the plant. Method: The dried grass of C.dactylon(20 kg) was extraced with 95% EtOH as the crude extract

and then suspended in water followed by extraction with petroleum ether

EtOAc and nBuOH to acquired four soluble extract successively. The compounds were isolated from the EtOAc extract(150 g) by successive chromatographic procedures(silica gel

RP-18

MCI

Sephadex LH-20

and Semi-preparative HPLC).Their structures were identified by physical and chemical properties and spectral data(1D NMR

2D NMR

UV

MS

CD

ORD). Result: Eight compounds were separated from C.dactylon and their structures were examined by spectral data and identified as 7-oxoarctigenin(1)

together with seven known compounds acetovanillone(2)

4-hydroxybenzoic acid(3)

(4-Hydroxy-3

5-diethoxyphenyl)(3-hydroxy-4-methoxy-phenyl)methanone(4)

2-hydroxybenzoicacid(5)

syringaresinol(6)

(5S

6S)-5

6-dihydro-3

8

10-trihydroxy-5-(4-hy-droxy-3-methoxyphenyl)-6-hydrox-ymethyl-2

4-dimethothoxy-7H-benzo[c]xanthen-7-one)(7)

and lunteolin(8). Conclusion: Compound 1-8 were isolated from C. dactylon for the first time.