Quality Evaluation of Cervi Cornu Pantotrichum Based on Multivariate Statistical Analysis

LIU Wei; GONG Wei; ZHANG Song; LI Ke-qiang; ZHANG Zhen-qiu; LI Feng

doi:10.13422/j.cnki.syfjx.20181418

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Quality Evaluation of Cervi Cornu Pantotrichum Based on Multivariate Statistical Analysis

更新时间：2024-01-04

- Quality Evaluation of Cervi Cornu Pantotrichum Based on Multivariate Statistical Analysis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 14, Pages: 57-62(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.20181418
  CLC： R284
- Published：2018
- 稿件说明：
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LIU Wei, GONG Wei, ZHANG Song, et al. Quality Evaluation of Cervi Cornu Pantotrichum Based on Multivariate Statistical Analysis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 57-62.
DOI：

LIU Wei, GONG Wei, ZHANG Song, et al. Quality Evaluation of Cervi Cornu Pantotrichum Based on Multivariate Statistical Analysis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(14): 57-62. DOI： 10.13422/j.cnki.syfjx.20181418.

摘要

目的：采用多元统计分析技术，探讨不同品种不同规格鹿茸的质量评价方法。方法：采用高效液相色谱法测定17种氨基酸、核苷组分、磷脂组分、胆固醇及多胺的含量；采用紫外分光光度法测定水溶性蛋白质及总磷脂含量；以测定的这些营养成分含量作为分析数据源，采用SPSS 19.0统计软件进行方差分析和聚类分析。结果：方差分析表明，不同规格及不同品种鹿茸之间氨基酸总量、总磷脂、磷脂组分、多胺无显著差异(P<0.05)，马鹿三岔中必需氨基酸的含量显著低于马鹿其他规格及梅花鹿所有样品的含量(P<0.05)，梅花鹿二杠侧枝二茬中核苷含量高于马鹿及梅花鹿二杠主枝头茬、三岔头茬，有显著性差异(P<0.05)，马鹿四岔中水溶性蛋白含量与其他规格马鹿、梅花鹿二杠主侧枝头茬及三茬有显著性差异(P<0.05)，马鹿单门及四岔胆固醇含量与梅花鹿二杠侧枝二茬、三岔有显著性差异(P<0.05)；聚类分析不能将马鹿茸和梅花鹿茸分为两大类，有交叉聚类现象，评价指标不同，聚类分析结果也不同。结论：应用方差分析技术及聚类分析法评价鹿茸的质量，具有可靠性，为鹿茸的开发和应用提供了重要的依据。

Abstract

Objective: To provide a reference for the quality control of the different species and specifications of Cervi Cornu Pantotrichum based on multivariate statistical analysis. Method: The contents of 17 amino acids

nucleoside

phospholipids

cholesterol and polyamine were determined by high performance liquid chromatography(HPLC). Ultraviolet spectrophotometry(UV) was employed for the determination of the contents of water soluble protein and total phospholipid. Variance analysis and cluster analysis were applied to study the characteristics of Cervi Cornu Pantotrichum based on the data of analysis of the content of nutrient content by SPSS 19.0. Result: There was not significantly difference in the content of total amino acid

total phospholipids

phospholipids and polyamines among different specifications and varieties of Cervi Cornu Pantotrichum(P<0.05). The content of essential amino acids in Sancha of Cervus elaphus was significantly lower than that of the other specifications of C. elaphus and all C. nippon(P<0.05). The content of nucleoside in Ergangcezhiercha of C. nippon was higher than that of Ergang zhuzhi toucha and Sancha toucha(P<0.05). The content of water-soluble protein in Sicha of C. elaphus was significantly different from that of other specifications of C. elaphus

Ergang zhucezhi toucha and Sancha of C. elaphuss(P<0.05). The variance analysis showed that the content of cholesterol in Danmen and Sicha of C. elaphus Linnaeus was significantly different from that of Ergangcezhiercha and Sancha of C. nippon(P<0.05). The clustering analysis could not classifiy C. elaphus and C. nippon into two main categories

because of the phenomenon of cross-clustering. Different evaluation indexes resulted in different results of cluster classification. Conclusion: The application of variance analysis technique and cluster analysis in evaluating the quality of Cervi Cornu Pantotrichum and its reliability is reliable

and provides important basis for the development and application of velvet antler.

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