Effect of Buyang Huanwu Tang in Stabilizing Vulnerable Plaques of ApoE Mice Atherosclerosis

LIU Yu-hui; HOU Bei-bei; YOU Yu; ZENG Lan-fang; XU Wei

doi:10.13422/j.cnki.syfjx.20181423

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Effect of Buyang Huanwu Tang in Stabilizing Vulnerable Plaques of ApoE Mice Atherosclerosis

更新时间：2024-01-04

- Effect of Buyang Huanwu Tang in Stabilizing Vulnerable Plaques of ApoE Mice Atherosclerosis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 15, Pages: 112-119(2018)
- 作者机构：
- 作者简介：
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- DOI：10.13422/j.cnki.syfjx.20181423
  CLC： R285.5
- Published：2018
- 稿件说明：
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LIU Yu-hui, HOU Bei-bei, YOU Yu, et al. Effect of Buyang Huanwu Tang in Stabilizing Vulnerable Plaques of ApoE Mice Atherosclerosis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(15): 112-119.
DOI：

LIU Yu-hui, HOU Bei-bei, YOU Yu, et al. Effect of Buyang Huanwu Tang in Stabilizing Vulnerable Plaques of ApoE Mice Atherosclerosis[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(15): 112-119. DOI： 10.13422/j.cnki.syfjx.20181423.

摘要

目的：探究补阳还五汤（BYHWT）对高脂饮食的载脂蛋白E敲除（ApoE-/-）小鼠动脉粥样硬化（atherosclerosis，AS）易损斑块的影响及其相关的作用机制。方法：8周龄ApoE-/-小鼠高脂饮食喂养12周复制AS易损斑块模型，以C57/BL-6J小鼠作为正常组给于正常饮食饮水。随机取4只小鼠中头臂动脉与主动脉弓制作病理切片，通过计算斑块面积及其易损指数判定模型复制成功。72只ApoE-/-小鼠随机分为6组，分别为正常组、模型组、自噬诱导剂组及BYHWT低、中、高剂量组，BYHWT低、中、高剂量组分别给予5，10，20 g·kg-1 BYHWT灌胃8周，自噬诱导剂组给予雷帕霉素4 mg·kg-1灌胃8周，正常组继续给于正常饮食饮水。8周后，小鼠处死，游离胸主动脉到腹主动脉末端，制备血管组织标本用于斑块的易损指数测定，采用逆转录聚合酶链式反应（RT-PCR）及蛋白免疫印迹法（Western blot）分析血管组织中自噬体形成。结果：ApoE-/-小鼠成功诱导AS易损斑块模型，模型组AS斑块形成明显，斑块易损指数显著大于正常组（P<0.01）；补阳还五汤各剂量组明显缩小斑块面积，易损指数与模型组比较有不同程度的降低（P<0.05）。模型组斑块血管组织中基质金属蛋白酶（MMP）-1，2，3 mRNA水平明显高于正常组；与模型组比较，补阳还五汤、雷帕霉素组的MMP-1，2，3 mRNA的水平明显降低（P<0.05，P<0.01）；与正常组比较，模型组的微管轻链蛋白3（LC3-Ⅱ/LC3-Ⅰ）含量显著降低，高、中、低剂量的补阳还五汤及其雷帕霉素组可以明显升高LC3-Ⅱ/LC-Ⅰ含量（P<0.05）。结论：补阳还五汤具有缩小AS斑块面积、降低血管中的基质金属蛋白酶的表达，调节斑块内巨噬细胞自噬从而降低斑块的易损指数，提高AS斑块的稳定性的作用。

Abstract

Objective: To investigate the effect of Buyang Huanwu Tang(BYHWT) on the vulnerable plaques in atherosclerosis in ApoE-/- mice with high-fat diet. Method:The 8-week-old ApoE-/- mice were fed with high-fat diet for 12 weeks to reproduce the atherosclerosis(AS) vulnerable plaque model

and C57/BL-6J mice were used as normal control group and given normal drinking water. A total of four mice were randomly selected to make pathological sections of the brachiocephalic arteries and the aortic arch. The successful modeling was verified by calculating the plaque area and its vulnerability index. 72 ApoE-/- mice were randomly divided into the following groups:model group

low-dose BYHWT group:5 g·kg-1 BYHWT for 8 weeks

middle-dose BYHWT group:10 g·kg-1 BYHWT for 8 weeks

high-dose BYHWT group:20 g·kg-1 BYHWT for 8 weeks

autophagy inducer group:rapamycin 4 mg·kg-1 for 8 weeks

control group:continue to give normal diet drinking water. After 8 weeks

the mice were put to death

and the thoracic aorta was dissociated to the end of the abdominal aorta. Some prepared vascular tissue samples were used for the determination of the vulnerability index of the plaque.PCR analysis and Western blot were used to analyze the formation of autophagosomes. Result:ApoE-/- mice were fed with high-fat diet for 16 weeks to successfully induce the AS vulnerable plaque model. The model group showed obvious AS plaque formation. The index of plaque vulnerability was significantly higher than that in the normal group (P<0.01). Each dose of BYHWT significantly reduced the plaque area

and the index of vulnerability was decreased to some extent compared with that of model group (P<0.05). The levels of matrix metalloproteinase(MMP)-1

MMP-2 and MMP-3 mRNA in the model group were significantly higher than those in the blank group. The levels of MMP-1

MMP-2 and MMP-3 mRNA in different doses of BYHWT and rapamycin group were significantly lower than those in model group (P<0.05

P<0.01). The level of LC3-Ⅱ/LC3-Ⅰ in the model group was significantly lower than that in the blank group; compared with the model group

different doses of BYHWT and rapamycin group significantly increased LC3-Ⅱ/LC3-Ⅰ level(P<0.05). Conclusion:BYHWT can reduce the area of AS plaque

decrease the expression of matrix metalloproteinase in blood vessel

and regulate macrophage autophagy in plaque

thus reducing the vulnerability index of plaque and improving the stability of AS plaque.

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Related Institution

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