Analysis of Integration of Processing and Processing of Producing Areas of Polygoni Multiflori Radix Based on Process Control

ZHENG Ying; LI Wei; ZHAO Gui; HOU Ming-yue; DU Hong-zhi; WU Shan-shan; QIU Ya-lei; ZHANG Yong-mei

doi:10.13422/j.cnki.syfjx.20181502

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Analysis of Integration of Processing and Processing of Producing Areas of Polygoni Multiflori Radix Based on Process Control

更新时间：2024-01-04

- Analysis of Integration of Processing and Processing of Producing Areas of Polygoni Multiflori Radix Based on Process Control
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 15, Pages: 29-35(2018)
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- DOI：10.13422/j.cnki.syfjx.20181502
  CLC： R283
- Published：2018
- 稿件说明：
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ZHENG Ying, LI Wei, ZHAO Gui, et al. Analysis of Integration of Processing and Processing of Producing Areas of Polygoni Multiflori Radix Based on Process Control[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(15): 29-35.
DOI：

ZHENG Ying, LI Wei, ZHAO Gui, et al. Analysis of Integration of Processing and Processing of Producing Areas of Polygoni Multiflori Radix Based on Process Control[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(15): 29-35. DOI： 10.13422/j.cnki.syfjx.20181502.

摘要

目的：比较产地加工与炮制一体化方法和传统炮制方法对何首乌中二苯乙烯苷、游离蒽醌类成分和总多糖含量的影响，为建立何首乌产地加工与炮制一体化方法提供依据。方法：采用一体化方法和传统炮制方法对何首乌饮片清蒸和黑豆汁拌蒸，在不同时间点取样，分别测定二苯乙烯苷、游离蒽醌类（大黄素、大黄素甲醚）和总多糖的含量。采用HPLC测定二苯乙烯苷和游离蒽醌类成分的含量，二苯乙烯苷含量测定的流动相为乙腈-水（25∶75），检测波长320 nm；游离蒽醌类成分含量测定的流动相为甲醇-0.1%磷酸水溶液（85∶15），检测波长254 nm；利用苯酚-硫酸法显色，运用UV测定总多糖含量，检测波长489 nm。结果：在同一时间取样，一体化方法与传统炮制方法对二苯乙烯苷含量均无显著性影响，随着蒸制时间的延长，何首乌传统炮制方法样品和一体化方法样品中二苯乙烯苷的质量分数呈逐渐降低的趋势；一体化方法与传统炮制方法对游离蒽醌类成分的含量具有显著性影响，且一体化方法中游离蒽醌类成分的含量明显低于传统炮制方法；2种炮制方法对总多糖含量均无显著性影响。结论：何首乌产地加工与炮制一体化方法制备的制何首乌质量较好、省时省力、可操作性强，具有一定的可行性。

Abstract

Objective: To compare the effects of integrated processing method and traditional processing method on the contents of stilbene glucoside

free anthraquinones and total polysaccharides in Polygoni Multiflori Radix(PMR)

and provide the basis for establishing integrated method of processing and processing of producing areas of PMR. Method: The contents of stilbene glucoside

free anthraquinones(emodin and physcione) and total polysaccharides were determined by sampling at different time points

PMR was steamed and steamed with black bean juice by integrated processing method or traditional processing method. Result: Sampling at the same time

the integrated processing method and traditional processing method had no significant effect on the content of stilbene glucoside

with the extension of steaming time

the content of stilbene glycoside in the samples of the traditional processing method and integrated processing method gradually decreased;the integrated processing method and traditional processing method had a significant effect on the content of free anthraquinones

the content of free anthraquinones in the integrated method was significantly lower than the traditional processing method;there are no significant effects of two kinds of processing methods on the content of total polysaccharides. Conclusion: The integrated method of processing and processing of producing areas of PMR is reasonable and feasible.

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