Objective:To observe the effect of Huatan Xiaoliu decoction (HXD) on tumor weight

lung metastases focuses

nm-23 and vascular endothelial growth factors(VEGF) in tumor tissues of Lewis tumor-bearing mice

and to explore its mechanism in restraining tumor growth and metastasis. Method:Totally 30 C57BL/6J male mice were randomly divided into the blank group

HXD group (50 g·kg-1·d-1)

and Cyclophosphamide (CTX) group[0.06 g·kg-1·(3

7 d)-1]

10 mice in each group. Lewis lung cancer cells were inoculated subcutaneously into right axillary of C57BL/6 mice to establish Lewis tumor-bearing mice models. After 21 days

the mice were killed

and the tumors were stripped to calculate the rates of inhibition; their lung tissues were taken and after fixing

the number of lung metastases focuses was observed under the microscope to calculate the rate of anti-lung metastasis. The mRNA expressions of VEGF and nm-23 were detected by Real-time PCR

and the protein expressions of VEGF and nm-23 were detected by Western blot. Result:In comparison with blank group

the tumor weight and the number of lung metastasis focuses were significantly decreased in HXD group and CTX group (P<0.01); the mRNA and protein expressions of VEGF were decreased

and the mRNA and protein expressions of nm-23 were increased in HXD group and CTX group. Conclusion:Huatan Xiaoliu decoction could inhibit Lewis lung cancer growth and metastasis

and the underlying mechanism may be related to reducing the expression of VEGF

reducing the generation of tumor new vasculature

up-regulating the expression of nm-23

and inhibiting tumor generation and metastasis.