Effect of Quercetin on Migration and Invasion Ability of U937 Cells and Expressions of MMP-2 and MMP-9

ZHU Ling-ling; ZHANG Yang; DOU Qin-ling; ZHANG Yun

doi:10.13422/j.cnki.syfjx.20181524

您当前的位置：

首页 >

文章列表页 >

Effect of Quercetin on Migration and Invasion Ability of U937 Cells and Expressions of MMP-2 and MMP-9

更新时间：2024-01-04

- Effect of Quercetin on Migration and Invasion Ability of U937 Cells and Expressions of MMP-2 and MMP-9
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 16, Pages: 146-151(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181524
  CLC： R285
- Published：2018
- 稿件说明：
移动端阅览
ZHU Ling-ling, ZHANG Yang, DOU Qin-ling, et al. Effect of Quercetin on Migration and Invasion Ability of U937 Cells and Expressions of MMP-2 and MMP-9[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(16): 146-151.
DOI：

ZHU Ling-ling, ZHANG Yang, DOU Qin-ling, et al. Effect of Quercetin on Migration and Invasion Ability of U937 Cells and Expressions of MMP-2 and MMP-9[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(16): 146-151. DOI： 10.13422/j.cnki.syfjx.20181524.

摘要

目的：观察槲皮素（quercetin）对人急性髓系白血病（human acute myeloid leukemia） U937细胞增殖、凋亡、黏附力、迁移和侵袭能力及基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2），基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）表达的影响。方法：体外培养U937细胞，分别以0，10，20，40 μmol·L-1槲皮素处理24，48，72 h，细胞计数试剂盒（cell counting kit-8，CCK-8）检测槲皮素对U937细胞增殖的抑制作用。实验随机分为空白组，槲皮素10，20，40 μmol·L-1组。末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法（TUNEL）检测U937细胞凋亡情况；细胞黏附实验检测U937细胞黏附力；划痕修复实验检测U937细胞迁移能力；transwell小室体外侵袭实验检测U937细胞侵袭能力；蛋白质印迹法（Western blot）检测U937细胞MMP-2，MMP-9蛋白表达。结果：槲皮素可抑制U937细胞的增殖。与空白组比较，槲皮素10，20，40 μmol·L-1组明显升高U937细胞凋亡指数（P<0.05，P<0.01）。与空白组比较，槲皮素10，20，40 μmol·L-1组明显降低U937细胞黏附率，迁移率，侵袭细胞数及MMP-2，MMP-9蛋白表达（P<0.05，P<0.01）。结论：槲皮素可显著抑制U937细胞迁移和侵袭能力，其机制可能与其抑制MMP-2和MMP-9表达有关。

Abstract

Objective: To investigate the Effect of quercetin on proliferation

apoptosis

adhesion

migration and invasion ability of U937 cells and expressions of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9). Method: U937 cells were cultured in vitro and treated with quercetin at various doses(0

40 μmol·L-1)for 24

72 h. The proliferation of U937 cells was measured by cell counting kit-8(CCK-8). U937 cells were randomly divided into blank group

quercetin 10 μmol·L-1 group

quercetin 20 μmol·L-1 group and quercetin 40 μmol·L-1 group. Apoptotic rates were evaluated by terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling(TUNEL). Adhesion of the cells was detected by the cell adhesion assay. Migration ability was evaluated by the wound healing assay. Invasion ability was evaluated by the transwell chamber assay. Expressions of MMP-2 and MMP-9 were detected by Western blot. Result: The proliferation of U937 cells was inhibited by quercetin. As compared with the blank group

quercetin 10 μmol·L-1 group

quercetin 20 μmol·L-1 group and quercetin 40 μmol·L-1 group significantly increased apoptotic indexes in U937 cells(P<0.05

P<0.01); and significantly decreased adhesion rates

migration rates

numbers of invasive cells

expressions of MMP-2 and MMP-9 in U937 cells(P<0.05

P<0.01). Conclusion: Quercetin could significantly inhibit the migration and invasion ability of U937 cells

and mechanism may be associated with down-regulating expressions of MMP-2 and MMP-9 in U937 cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Jinlong Capsule on Invasion and Migration of Human Gastric Carcinoma MGC-803 and BGC-823 Cells

Effect of Ginsenoside Rh₂ on Metastasis and Invasion of Colorectal Cancer Resistant Cells HCT116/L-OHP and Its Mechanism

Effect of Astragaloside Ⅳ on Proliferation， Migration， and Invasion of Colorectal Cancer HCT116 Cells

Mechanism of Tumor Suppressor miRNAs in Migration and Invasion of Nasopharyngeal Carcinoma and Intervention of Chinese Medicine： A Review

Effect of Total Flavone of Litchi Semen on Proliferation， Migration， and Invasion of HepG2 Cells Based on JAK2/STAT3 Signaling Pathway

Related Author

LI Dan

JIN Feng

TAO Li

NI Teng-yang

WANG Hai-bo

FENG Jun

ZHU Guang

QIAN Ya-yun

Related Institution

Shaanxi University of Chinese Medicine

Xijing Hospital of Digestive Disease, Air Force Medical University

Hospital Affiliated to Shaanxi University of Chinese Medicine

Xianyang Central Hospital

The First Affiliated Hospital of Nanchang University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰