Optimization of Processing Technology of Atractylodis Macrocephalae Rhizoma Processed with Aurantii Fructus Immaturus and Analysis of Its HPLC Fingerprint

WANG Fang; YU Huan; LI Feng-qin; ZHANG Shuai-jie; LIU Huai-wei; DAI Liang-min; LI Yan; YANG Ming

doi:10.13422/j.cnki.syfjx.20181604

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Optimization of Processing Technology of Atractylodis Macrocephalae Rhizoma Processed with Aurantii Fructus Immaturus and Analysis of Its HPLC Fingerprint

更新时间：2024-01-04

- Optimization of Processing Technology of Atractylodis Macrocephalae Rhizoma Processed with Aurantii Fructus Immaturus and Analysis of Its HPLC Fingerprint
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 17, Pages: 6-13(2018)
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- DOI：10.13422/j.cnki.syfjx.20181604
  CLC： R283
- Published：2018
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WANG Fang, YU Huan, LI Feng-qin, et al. Optimization of Processing Technology of Atractylodis Macrocephalae Rhizoma Processed with Aurantii Fructus Immaturus and Analysis of Its HPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(17): 6-13.
DOI：

WANG Fang, YU Huan, LI Feng-qin, et al. Optimization of Processing Technology of Atractylodis Macrocephalae Rhizoma Processed with Aurantii Fructus Immaturus and Analysis of Its HPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(17): 6-13. DOI： 10.13422/j.cnki.syfjx.20181604.

摘要

目的：探讨枳实制白术的最佳炮制工艺，建立其HPLC特征图谱。方法：采用"脾虚泄泻"免疫力低下动物模型和大鼠饮水量来评价枳实制白术的健脾祛燥作用；通过对比不同配比枳实制白术饮片的健脾祛燥作用，筛选枳实与白术的最佳配比；采用正交试验，以辛弗林和白术内酯Ⅲ的含量为评价指标，优选枳实制白术的炮制工艺，并建立其特征图谱。结果：10%枳实制白术组的效果最佳。枳实制白术饮片炮制过程中，影响辛弗林及白术内酯Ⅲ的主次因素依次为烘制温度 > 炒制温度 > 炒制时间，最佳炮制工艺为取生白术饮片100 g，加入枳实汁50 mL（生药质量浓度0.2 g·mL-1），浸泡24 h，不时翻动，直至汁尽药透，于110℃炒制6 min，取出，于40℃烘箱干燥。枳实制白术有5个共有峰，均能达到较好分离，该分析方法具有良好的精密度、重复性和稳定性，符合特征图谱相关要求。结论：药汁制是中药炮制的重要组成部分，枳实制白术的健脾祛燥作用充分体现了这一炮制特色——增效，纠正偏性。建立的HPLC特征图谱方法准确、可靠，为枳实制白术的质量控制提供了参考。

Abstract

Objective:To optimize processing technology of Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus and establish its HPLC fingerprint. Method:The invigorating spleen and dispelling dryness of Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus was investigated by model rats with splenasthenic diarrhea

and then the best ratio of Atractylodis Macrocephalae Rhizoma and Aurantii Fructus Immaturus was screened.Taking overall desirability of contents of synephrine and atractylenolide Ⅲ as index

orthogonal test was employed to optimize processing technology

and HPLC fingerprint of this processed product was established. Result:The best ratio of Atractylodis Macrocephalae Rhizoma to Aurantii Fructus Immaturus was 9:1.The factors influenced contents of synephrine and atractylenolide Ⅲ were followed by baking temperature > frying temperature > frying time

optimum processing technology of this processed product was as follows:added Aurantii Fructus Immaturus juice 50 mL(crude drug concentration of 0.2 g·mL-1) into 100 g of Atractylodis Macrocephalae Rhizoma pieces

soaked 24 hours

fried 6 min at 110℃

dried at 40℃ in a oven.Five common peaks were selected in Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus.Among the obtained fingerprint

all of the detected peaks were separated effectively.The accuracy

repeatability and stability of this method were satisfied. Conclusion:The invigorating spleen and dispelling dryness of Atractylodis Macrocephalae Rhizoma processed with Aurantii Fructus Immaturus can reflect the processing characteristics of increasing efficiency and correcting bias.The established HPLC fingerprint can provide reference for the quality control of this processed product.

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Related Institution

The Third Affiliated Hospital of Henan University of Chinese Medicine

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TCM Processing Technology Inheritance Base of the State Administration of TCM

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