Comprehensive Evaluation of Quality of Erzhiwan Based on Five Characteristic Components

FAN Miao-xuan; TAN Cheng; WANG Meng-meng; DU Xiao-wei; LIU Ying; WANG Jing-hui; CHEN You-gen; FU Xin-tong

doi:10.13422/j.cnki.syfjx.20181607

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Comprehensive Evaluation of Quality of Erzhiwan Based on Five Characteristic Components

更新时间：2024-01-04

- Comprehensive Evaluation of Quality of Erzhiwan Based on Five Characteristic Components
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 17, Pages: 88-92(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181607
  CLC： R286.0
- Published：2018
- 稿件说明：
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FAN Miao-xuan, TAN Cheng, WANG Meng-meng, et al. Comprehensive Evaluation of Quality of Erzhiwan Based on Five Characteristic Components[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(17): 88-92.
DOI：

FAN Miao-xuan, TAN Cheng, WANG Meng-meng, et al. Comprehensive Evaluation of Quality of Erzhiwan Based on Five Characteristic Components[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(17): 88-92. DOI： 10.13422/j.cnki.syfjx.20181607.

摘要

目的：建立二至丸中5种特征性成分特女贞苷、红景天苷、酪醇、木犀草素、蟛蜞菊内酯同时检测的含量测定方法，并据此评价所收集的11批二至丸样品的质量。方法：采用Phenomenex Gemini C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈（A）-0.2%甲酸水溶液（B）梯度洗脱（0~15 min，10%~30% A；15~25 min，30%~33% A；25~30 min，33% A）。流速1 mL·min-1，检测波长225 nm和349 nm，柱温30℃。结果：5个成分的分离度良好，红景天苷、酪醇、特女贞苷、木犀草素和蟛蜞菊内酯的线性范围依次为202.55~12 153，84.55~5 073，244.30~14 657.72，2.56~153.72，2.32~139.2 ng。加样回收率平均值处于95.61%~103.47%，RSD均<2.0%。结论：建立的含量测定方法简便、准确，重复性好，可用于市售二至丸样品的含量测定和质量控制。11批样品中5个特征性成分含量差异较大，特女贞苷含量差异>60倍，最低值0.15 mg·g-1，最高值10.22 mg·g-1，其原因可能与原料质量和执行标准等因素有一定关系。

Abstract

Objective:To evaluate the quantity of 11 batches of Erzhiwan samples by establishing a HPLC method for simultaneous determination of five characteristic components(specnuezhenide

salidroside

tyrosol

luteolin and wedelolactone) in this preparation. Method:Phenomenex Gemini C18 column(4.6 mm×250 mm

5 μm) was employed with mobile phase of acetonitrile-0.2% formic acid solution for gradient elution(0-15 min

10%-30%A;15-25 min

30%-33%A;25-30 min

33%A)

flow rate was 1 mL·min-1

detection wavelengths were 225 nm and 349 nm

column temperature was set at 30℃. Result:Salidroside

tyrosol

specnuezhenide

luteolin and wedelolactone were well separated with linear ranges of 202.55-12 153

84.55-5 073

244.30-14 657.72

2.56-153.72

2.32-139.2 ng

respectively;their average recovery rates were between 95.61% to 103.47% with RSD<2.0%. Conclusion:This determination of five components is simple

accurate and reproducible for the quality control of Erzhiwan.The contents of these five components in 11 batches of samples are quite different.The content of specnuezhenide in samples is ranged from 0.15 mg·g-1 to 10.22 mg·g-1

which may be caused by the materials

the operative norms and other factors.

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