GUO Na, XIAO Yun-feng, ZHANG Yuan-yan, et al. Metabolomics of Roudoukou-8 San in Preprotecting Myocardial Ischemia Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(16): 67-72.
DOI:
GUO Na, XIAO Yun-feng, ZHANG Yuan-yan, et al. Metabolomics of Roudoukou-8 San in Preprotecting Myocardial Ischemia Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(16): 67-72. DOI: 10.13422/j.cnki.syfjx.20181609.
Metabolomics of Roudoukou-8 San in Preprotecting Myocardial Ischemia Reperfusion Injury
Objective: To study on the changes of endogenous metabolites in the urine of rats with myocardial ischemia reperfusion injury(MIRI) after administrating Roudoukou-8 San
in order to explore the pathogenesis of MIRI and the mechanism of drug pre-protection. Method: Rats were randomly divided into the sham-operated group[0.5% sodium carboxymethylcellulose(CMC-Na) solution
10 mL·kg -1]
the model group(0.5% CMC-Na
10 mL·kg -1) and the Roudoukou-8 San group(5.4 mg·kg-1).The urine metabolites of these three groups were characterized by UPLC-Q-Exactive-MS technique.Multivariate statistical analysis was performed by SIMCA 14.1 software to screen potential biomarkers. Result: After orthogonal partial least squares discriminant analysis(OPLS-DA)
the model group was significantly separated from the sham-operated group and the Roudoukou-8 San group
partial areas of the Roudoukou-8 San group overlapped with and the sham-operated group.Eight biomarkers related to MIRI were screened and they correlated with amino acid metabolism and fatty acid metabolism. Conclusion: Roudoukou-8 San can effectively regulate the imbalance of amino acid metabolism and fatty acid metabolism related to urine metabolism in MIRI model rats and preliminarily protect MIRI through multiple pathways.
Analysis of Chemical Composition in Puerariae Thomsonii Stem Base and Investigation of Its Effect on Liver Metabolites in Mice Based on UPLC-Q-TOF-MS/MS
Investigation of Mechanism of Qingqiao and Laoqiao on Acute Lung Injury Rats Based on Serum Metabolomics
Exploring Central Regulatory Effect of Chaishao Liujuntang on Chronic Atrophic Gastritis Rats with Liver Depression and Spleen Deficiency Syndrome Based on Metabolomics
Metabolomic Analysis of Urine in Rat Model with Spleen-stomach Damp-heat Syndrome
Analysis of Mechanism of Artesunate in Treatment of Liver Cancer Based on Metabolomics
Related Author
MENG Lingbang
YU Huan
WU Xiaoying
GUO Yijing
QU Fei
GONG Qianfeng
LI Kang
LYU Tiantian
Related Institution
School of Pharmacy, Jiangxi University of Chinese Medicine
Yongxiu Administration for Market Regulation
Hebei Mental Health Center
School of Pharmacy, Henan University of Chinese Medicine