Objective:To identify the differentially expressed proteins in skeletal muscle of type 2 diabetes KKAy mice from the model group and the administration group by nano-LC-LTQ/Orbitrap-MS

and speculate mechanism of couplet medicines of Scutellariae Radix and Coptidis Rhizoma against type 2 diabetes. Method:Twenty male KKAy mice aged 8-10 weeks were used. KKAy mice were given full-fledged high-fat diet and randomly divided into 2 groups

included Scutellariae Radix and Coptidis Rhizoma group(27 g·kg-1) and model group(the same amount of distilled water).Mice in the two groups were continuous intragastric administrated for 8 weeks.After the last administration for 1 hour

mice were sacrificed

skeletal muscle was taken and placed in -80℃ refrigerator.Skeletal muscles of the model group and administration group were ground in liquid nitrogen

lysate and protease inhibitors were added to extract the protein

the concentration of protein was determined

and then protein denaturation

reduction

alkylation

enzymolysis and desalting

the samples were identified by nano-LC-LTQ/Orbitrap-MS.Data were processed by Xcalibur 2.2 software and Proteome Discoverer 1.4.0.288

and Sieve v2.1(×64) was adopted to analyze and find relevant differential proteins with quantitative analysis of non-standard samples

differentially expressed proteins were screened and identified

and bioinformatics analysis of the differentially expressed proteins was carried out. Result:A total of 107 differentially expressed proteins were identified from Scutellariae Radix and Coptidis Rhizoma group and model group

and five differential proteins related to diabetes were screened out by biological analysis

including 4 up-regulated[5'-adenosine-activated protein kinase(AMPK)

adiponectin(APN)

etc] and 1 down-regulated[adipocyte type fatty acid binding protein4(FABP4)].The differential proteins mainly involved in inflammatory reactions

signal pathway regulation and other biological processes. Conclusion:Through effective comparison and analysis

we obtain some differentially expressed proteins involved in insulin signal transduction and other pathways

this paper can provide a new target for the study on the molecular mechanism of Scutellariae Radix and Coptidis Rhizoma against insulin resistance.