Protective Effect of Bacuchiol on Hepatic Stellate Cells Induced by TGF-

CHEN Qiao-yun; YANG Liu; WANG Ye-qiu; LI Meng; ZHANG Jian-hui; LI Jian-min

doi:10.13422/j.cnki.syfjx.20181624

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Protective Effect of Bacuchiol on Hepatic Stellate Cells Induced by TGF-

更新时间：2024-01-04

- Protective Effect of Bacuchiol on Hepatic Stellate Cells Induced by TGF-
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 16, Pages: 131-135(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.20181624
  CLC： R285
- Published：2018
- 稿件说明：
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CHEN Qiao-yun, YANG Liu, WANG Ye-qiu, et al. Protective Effect of Bacuchiol on Hepatic Stellate Cells Induced by TGF-[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(16): 131-135.
DOI：

CHEN Qiao-yun, YANG Liu, WANG Ye-qiu, et al. Protective Effect of Bacuchiol on Hepatic Stellate Cells Induced by TGF-[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(16): 131-135. DOI： 10.13422/j.cnki.syfjx.20181624.

摘要

目的：探讨补骨脂酚对转化生长因子-β（TGF-β）诱导人肝星状细胞损伤的保护作用及其机制。方法：通过噻唑蓝（MTT）比色法检测各组人肝星状细胞活性，筛选出补骨脂酚安全有效浓度；本实验分为空白组、模型组、补骨脂酚组（1×10-5mol·L-1）及雌二醇组（1×10-6mol·L-1），同样检测各组细胞的活性，除空白组外，其余各组均加入10 μg·L-1 TGF-β诱导人肝星状细胞损伤；分别采用酶联免疫吸附测定法（ELISA）检测各组细胞中超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GSH-Px），丙二醛（MDA）的水平；实时荧光定量聚合酶链式反应（Real-time PCR）检测细胞中基质金属蛋白酶-1（MMP-1），胶原蛋白-Ⅰ（Col-Ⅰ） mRNA表达水平；蛋白免疫印迹法（Western blot）检测细胞中MMP-1，Col-Ⅰ蛋白的表达水平。结果：与模型组比较，1×10-5 mol·L-1补骨脂酚显著提高TGF-β诱导人肝星状细胞及细胞中SOD，GSH-Px活性，显著降低细胞中MDA含量及Col-Ⅰ，MMP-1 mRNA和蛋白的表达（P<0.01）。结论：补骨脂酚对TGF-α诱导人肝星状细胞损伤的保护作用，主要是通过提高细胞中抗氧化酶的活性、降低细胞中MMP-1，Col-Ⅰ mRNA和蛋白的含量。

Abstract

Objective: To study the protective effect of bacuchiol on hepatic stellate cells induced by transform growth factor-β(TGF-β) and its mechanism. Method: The activity of each group of cells was detected by methye thiazolye telrazlium(MTT)

in order to screen out the safe and effective concentration of bacuchiol. This experiment was divided into blank group

model group (10 μg·L-1 TGF-β)

bacuchiol group (1×10-5 mol·L-1)

estradiol group (1×10-6 mol·L-1)

and their activities were detected. Superoxide dismutase (SOD)

Glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) of each group were detected by enzyme linked immunosorbent assay(ELISA); Real-time quantitative-PCR (Real-time PCR) was used to detect the changes in matrix metalloproteinase-1 (MMP-1) and collagen-Ⅰ (Col-Ⅰ) mRNA expressions in hepatic stellate cells induced by TGF-β; Western blot method was used to detect the changes of MMP-1 and Col-Ⅰ protein content in hepatic stellate cells induced by TGF-β. Result: The 1×10-5mol·L-1 bacuchiol can effectively improve the activity of SOD and GSH-Px in hepatic stellate cells

and also reduce MDA content in cells and the expressions of Col-Ⅰ and MMP-1 mRNA and protein (P<0.01). Conclusion: Bacuchiol has a protective effect on hepatic stellate cells induced by TGF-β mainly by improving the activity of antioxidant enzymes in cells and reducing the content of MMP-1

Col-Ⅰ mRNA and protein expressions in cells.

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