Effect of Aconiti Lateralis Radix Praepareta Combined with Glycyrrhizae Radix et Rhizoma on Intestinal Absorption of Alkaloids in Aconiti Lateralis Radix Praepareta
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Effect of Aconiti Lateralis Radix Praepareta Combined with Glycyrrhizae Radix et Rhizoma on Intestinal Absorption of Alkaloids in Aconiti Lateralis Radix Praepareta
Chinese Journal of Experimental Traditional Medical FormulaeVol. 24, Issue 22, Pages: 11-16(2018)
Li Wen, YANG Xiao-qin, ZHANG Jin-ming, et al. Effect of Aconiti Lateralis Radix Praepareta Combined with Glycyrrhizae Radix et Rhizoma on Intestinal Absorption of Alkaloids in Aconiti Lateralis Radix Praepareta[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 11-16.
DOI:
Li Wen, YANG Xiao-qin, ZHANG Jin-ming, et al. Effect of Aconiti Lateralis Radix Praepareta Combined with Glycyrrhizae Radix et Rhizoma on Intestinal Absorption of Alkaloids in Aconiti Lateralis Radix Praepareta[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 11-16. DOI: 10.13422/j.cnki.syfjx.20181704.
Effect of Aconiti Lateralis Radix Praepareta Combined with Glycyrrhizae Radix et Rhizoma on Intestinal Absorption of Alkaloids in Aconiti Lateralis Radix Praepareta
Objective:To study on the intestinal absorption of six alkaloids in Aconiti Lateralis Radix Praepareta(ALRP)-Glycyrrhizae Radix et Rhizoma(GRR) before and after their compatibility by in situ perfusion method in rats. Method:A rat intestinal perfusion model was used
the mass concentration of phenol red was measured by ultraviolet spectrophotometry
and the contents of aconitine
hypaconitine
mesaconitine
benzoylaconine
benzoylhypaconine
benzoylmesaconine in the perfusate were measured by LC-MS.Effect of three modes of compatibility on intestinal absorption of six alkaloids in ALRP decoction
ALRP-GRR combined decoction and ALRP-GRR separated decoction were investigated. Result:Compared with ALRP decoction
absorption rate constant(Ka) and absorption rate per unit time(A) of benzoylaconine in ALRP-GRR combined decoction and ALRP-GRR separated decoction showed a downward trend.Compared with ALRP-GRR separated decoction
Ka and A of benzoylmesaconine in ALRP-GRR combined decoction and ALRP decoction was decreased.Compared with ALRP decoction
Ka and A of hypaconitine in ALRP-GRR combined decoction showed a decreasing trend
Ka and A of hypaconitine in ALRP-GRR separated decoction showed a increasing trend.Compared with ALRP decoction
absorption of benzoylhypacoitine
aconitine and mesaconitine in ALRP-GRR combined decoction was in decline. Conclusion:Absorption of six alkaloids from ALRP in duodenum of rats decreases to some extent after combined with GRR.This study provides an experimental basis for the interpretation of the compatibility theory of "ALRP used with GRR can be absorbed slowly".