Objective: To establish UPLC-MS analysis method for the determination of dendrobine in the main organs of C57BL/6J mice

and explore the tissue distribution characteristics of dendrobine in vivo. Method: At 0

30

60

120 min after gavage

the main organs (heart

liver

spleen

lung

kidney

brain and intestine) were collected and precipitated by acetonitrile

and the supernatant was detected with UPLC-MS.The chromatography separation was performed on a Hypersil Gold C18 column(2.1 mm×150 mm

1.9 μm) with acetonitrile-0.1% formic acid in water for gradient elution.Heated electrospray ionization source(HESI) was applied and operated in the positive ion mode.Meanwhile

pseudoephedrine hydrochloride was used as the internal standard for determining. Result: The endogenous substances in the tissues of mice had no interference for the assay of dendrobine

and the methodological study met the requirements.Dendrobine could distribute in heart

liver

spleen

lung

kidney

brain and intestine tissue of mice

and its content reached the peak of content at 30 min after gavage

and then decreased significantly with time.The distributions of dendrobine at different time points after administration were distinct.Dendrobine was mainly distributed in liver tissue at 30 min (1 241.69±78.64) μg·L-1

and in jejunum and ileum at 60

120 min (166.15±10.80) μg·L-1 and (118.25±8.51) μg·L-1

respectively. Conclusion: Dendrobine is widely distributed in mice

and its concentration in intestinal tissue and liver is high.The distribution in brain suggests that dendrobine can penetrate the blood-brain barrier

and this study can provide a basis for elucidating its neuropharmacological action.