Objective:To study the pollen viability and stigma receptivity of Polygala tenuifolia

so as to provide a scientific basis for sexual reproduction and breeding of new varieties. Method:The pollen viability of different blooming days (the day before flowering

day 1

day 2

day 3

day 4

day 5

day 6

day 7

day 8) were determined by the I2-KI method and the TTC method

and the staining effect of the two staining methods on pollen viability were compared. I2-KI method was used to determine the pollen activity of Polygala tenuifolia pollens in different periods of time

different storage methods (wet preserved flowers and dry preserved flowers) and different storage conditions (4

26

-20

-80℃) in different periods of time (2

4

8

24

48

72 h). And its stigma receptivity was estimated by the benzidine-hydrogen peroxide. Result:①The pollen viability of P. tenuifolia decreased with the rise in the powder loosing time

the highest pollen viability was observed on the 4th day after flowering;②from 8:00 to 18:00 during the daytime

the pollen viability between 10:00 and 16:00 was stronger;③dry pollen can prolong the storage time under the condition of -20℃;④ at the 5th day after flowering

the stigma receptivity was the strongest. Conclusion:Pollen on the 4th day after flowering can be collected

and artificial pollination can be adopted for stigma on the 5th day after flowering stigma artificial pollination

so as to provide a theoretical basis for breeding of P. tenuifolia.