Analysis and Identification of Chemical Components in Trichosanthis Fructus by UPLC-LTQ-Orbitrap-MS

Xin LI; Li-ying TANG; Jing XU; Xian-kuo YU; Hong-jun YANG; De-feng LI; Yi ZHANG; Jian-wei FAN; Rui-qiang SU; Hong-wei WU; Qiong ZHANG

doi:10.13422/j.cnki.syfjx.20181715

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Analysis and Identification of Chemical Components in Trichosanthis Fructus by UPLC-LTQ-Orbitrap-MS

Resource and Quality Evaluation | 更新时间：2021-02-09

- Analysis and Identification of Chemical Components in Trichosanthis Fructus by UPLC-LTQ-Orbitrap-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 1, Pages: 201-210(2019)
- 作者机构：
  
  1.山西医科大学　药学院，太原　 030001
  2.中国中医科学院　中药研究所，北京　 100700
  3.鲁南制药集团股份有限公司　中药制药共性技术国家重点实验室，山东　临沂　 276006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181715
  CLC： R284.1;R289;R22
- Received：27 February 2018，
  
  Published Online：14 June 2018，
  
  Published：05 January 2019
- 稿件说明：
移动端阅览
Xin LI, Li-ying TANG, Jing XU, et al. Analysis and Identification of Chemical Components in Trichosanthis Fructus by UPLC-LTQ-Orbitrap-MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 201-210.
DOI：

Xin LI, Li-ying TANG, Jing XU, et al. Analysis and Identification of Chemical Components in Trichosanthis Fructus by UPLC-LTQ-Orbitrap-MS[J]. Chinese journal of experimental traditional medical formulae, 2019, 25(1): 201-210. DOI： 10.13422/j.cnki.syfjx.20181715.

摘要

目的：

基于超高效液相色谱-线性离子阱-静电场轨道阱高分辨质谱系统（UPLC-LTQ-Orbitrap-MS）分析鉴定中药瓜蒌的化学成分。

方法：

瓜蒌样品粉碎过筛后采用70%甲醇超声提取，Waters HSS T3-C

色谱柱（2.1 mm×100 mm，1.8 μm），流动相0.1%甲酸水溶液-甲醇梯度洗脱，流速0.3 mL·min

－1

，采用电喷雾离子源（ESI），正、负离子分别进行一级、二级质谱数据采集。为了获得更多的化合物信息，根据色谱峰分离情况、质谱响应强度以及一级质谱解卷积后提取得到的准分子离子数量，优化样品提取条件、色谱及质谱参数。根据精确相对分子质量、离子碎片信息、相关对照品比对，并结合瓜蒌化学成分文献信息，对其中的化学成分进行分析鉴定。

结果：

从瓜蒌中共分析鉴定了91个化学成分，包括14个氨基酸类化合物，5个单萜类化合物，5个四环三萜类化合物，1个五环三萜类化合物，14个黄酮类化合物，17个有机酸类化合物，3个多糖类化合物，7个核苷类化合物，7个生物碱及含氮化合物，12个挥发性成分，1个植物甾醇，及其他5种化合物。

结论：

该方法具有高效分离、高灵敏检测优势，可较为全面地对瓜蒌中不同类型化合物进行分析鉴定，为瓜蒌药效物质基础及质量标准研究提供参考。

Abstract

Objective:

To analysis and identify the chemical components in Trichosanthis Fructus by UPLC-LTQ-Orbitrap-MS.

Method:

Samples of Trichosanthis Fructus were extracted by ultrasonic with 70% methanol after smashing and sifting by 40 mesh sieve. Thermo Scientific™ Dionex™ UltiMate™ 3000 Rapid Separation LC system performed UPLC separations with Waters HSS T3-C

(2.1 mm×100 mm

1.8 μm) column. The mobile phase was 0.1% formic acid water(A)-methanol(B)with a gradient elution. The volume flow was 0.3 mL·min

-1

. A Thermo Scientific™ LTQ-Orbitrap mass spectrometer equipped with a ESI probe was employed. The samples were respectively scanned in MS

and MS

mode of positive and negative ions. According to the chromatographic peak separation

mass signal intensity

and the number of molecular ions in MS

model

the extraction condition

chromatogram and mass spectrum parameters were optimized. The chemical compounds were identified by the accurate mass measurement of molecular ions and fragment ion and comparation with reference substance.

Result:

91 chemical compositions in Trichosanthis Fructus were totally identified

including 14 amino acids

5 monoterpenoids

5 tetracyclic triterpenoids

1 pentacyclic triterpene

14 flavonoids

17 organic acids

3 polysaccharides

7 nucleotides

7 alkaloids and nitrogen compounds

2 volatile components

1 phytosterol

5 other compositions.

Conclusion:

The established UPLC-LTQ-Orbitrap-MS method can be used to quickly analyze and identify the main chemical constituents of Trichosanthis Fructus. The chemical information concerning the constituents in Trichosanthis Fructus could be helpful to the quality control and further studies of Trichosanthis Fructus.

关键词

Keywords

references

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