Effect of Curcumol Combined with 5-fluorouracil on Proliferation and Apoptosis of Colorectal Carcinoma Cell Line LoVo

LIU Hao-wei; WANG Juan; QIN Jian-li; LI Xu-mei; CHEN Xu

doi:10.13422/j.cnki.syfjx.20181717

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Effect of Curcumol Combined with 5-fluorouracil on Proliferation and Apoptosis of Colorectal Carcinoma Cell Line LoVo

更新时间：2024-01-04

- Effect of Curcumol Combined with 5-fluorouracil on Proliferation and Apoptosis of Colorectal Carcinoma Cell Line LoVo
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 18, Pages: 130-133(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181717
  CLC： R285.5
- Published：2018
- 稿件说明：
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LIU Hao-wei, WANG Juan, QIN Jian-li, et al. Effect of Curcumol Combined with 5-fluorouracil on Proliferation and Apoptosis of Colorectal Carcinoma Cell Line LoVo[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(18): 130-133.
DOI：

LIU Hao-wei, WANG Juan, QIN Jian-li, et al. Effect of Curcumol Combined with 5-fluorouracil on Proliferation and Apoptosis of Colorectal Carcinoma Cell Line LoVo[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(18): 130-133. DOI： 10.13422/j.cnki.syfjx.20181717.

摘要

目的：探讨莪术醇（curcumol）联合5-氟尿嘧啶（5-Fu）对大肠癌LoVo细胞增殖和凋亡作用的影响，以及药物处理后，LoVo细胞中增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）和凋亡相关蛋白B淋巴细胞瘤-2（B cell lymphoma-2，Bcl-2）表达量的变化情况。方法：莪术醇（50 mg·L-1）单独或联合5-Fu （2 mg·L-1）作用大肠癌LoVo细胞，用噻唑蓝（MTT）比色法检测各组药物对细胞增殖的抑制作用，采用流式细胞术分析各组对细胞凋亡作用的影响，应用蛋白免疫印迹法（Western blot）检测各组细胞中，增殖和凋亡相关蛋白PCNA，Bcl-2蛋白表达的变化情况。结果：与空白组比较，莪术醇，5-Fu单独给药以及联合用药，均可以有效抑制大肠癌LoVo细胞的增殖，促进细胞凋亡（P<0.05），同时可以降低PCNA，Bcl-2蛋白的表达（P<0.05）；与单独给药组比较，联合用药后，凋亡的促进作用更为明显（P<0.05）。与空白组比较，各药物组可以更有效的抑制PCNA，Bcl-2蛋白的表达（P<0.05）。结论：莪术醇联合5-氟尿嘧啶能有效抑制人大肠癌LoVo细胞的增殖和凋亡，与单独用药组比较，联合用药可以使癌细胞的敏感性增强，极大程度的促进人大肠癌LoVo细胞凋亡，且这些机制可能与下调PCNA，Bcl-2蛋白表达有关。

Abstract

Objective: To investigate the effect of curcumol combined with 5-fluorouracil (5-Fu) on the proliferation

apoptosis and the changes in the expressions of proliferating cell nuclear antigen (PCNA)

B cell lymphoma-2 (Bcl-2) in colorectal carcinoma cell line LoVo. Method: LoVo cells were treated with curcumol (50 mg·L-1) and/or 5-Fu (2 mg·L-1). The proliferation of LoVo cells were detected by methylthiazolyldiphenyl-tetrazolium bromide (MTT)assay. The apoptosis were analyzed by flow cytometer. The changes in PCNA and Bcl-2 expressions were detected by Western blot. Result: Curcumol

5-Fu and combination group could inhibit the proliferation and promote the apoptosis of LoVo cells. The rates of inhibition and apoptosis were significantly different from control group (P<0.05). And the expressions of PCNA and Bcl-2 were obviously decreased

with a significant difference from control group (P<0.05). After combined treatment

the promoting effect of apoptosis was more obvious than single treatment group (P<0.05)

and the expressions of PCNA and Bcl-2 protein were significantly decreased

with a significant difference from control group (P<0.05). Conclusion: Curcumol combined with 5-Fu could effectively inhibit the proliferation and promote the apoptosis of LoVo cells. Compared with the single treatment group

the combination of curcumol and 5-Fu can increase the sensitivity of cancer cells and greatly promote the apoptosis of LoVo cells. These mechanisms may be related to the down-regulation of PCNA and Bcl-2 protein expressions.

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