Effect and Mechanism of Capsaicin on Migration and Invasion of Hepatoma SMMC-7721 Cells

XIAO Chan-chan; CHEN Mao-jian; MEI Fan-biao; FANG Xiang; HUANG Tian-ren; LI Ji-lin; DENG Wei

doi:10.13422/j.cnki.syfjx.20181718

您当前的位置：

首页 >

文章列表页 >

Effect and Mechanism of Capsaicin on Migration and Invasion of Hepatoma SMMC-7721 Cells

更新时间：2024-01-04

- Effect and Mechanism of Capsaicin on Migration and Invasion of Hepatoma SMMC-7721 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 18, Pages: 124-129(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181718
  CLC： R285.5
- Published：2018
- 稿件说明：
移动端阅览
XIAO Chan-chan, CHEN Mao-jian, MEI Fan-biao, et al. Effect and Mechanism of Capsaicin on Migration and Invasion of Hepatoma SMMC-7721 Cells[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(18): 124-129.
DOI：

XIAO Chan-chan, CHEN Mao-jian, MEI Fan-biao, et al. Effect and Mechanism of Capsaicin on Migration and Invasion of Hepatoma SMMC-7721 Cells[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(18): 124-129. DOI： 10.13422/j.cnki.syfjx.20181718.

摘要

目的：观察辣椒碱（capsaicin）对肝癌SMMC-7721细胞迁移和侵袭的影响及可能的分子机制。方法：设立不同浓度辣椒碱组以及空白组，采用细胞计数试剂盒-8法（CCK-8）检测辣椒碱（0，25，50，75，100，150，200，250 μmol·L-1）处理肝癌SMMC-7721细胞24 h后的细胞活性；穿透小室（transwell）检测辣椒碱（0，50，75，100 μmol·L-1）的肝癌SMMC-7721细胞迁移和侵袭能力；蛋白质免疫印迹法（Western blot）检测辣椒碱（0，50，75，100 μmol·L-1）处理肝癌SMMC-7721细胞24 h后，对细胞中的E-钙黏蛋白（E-cadherin），波形蛋白（Vimentin），基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）和（matrix metalloproteinase-9，MMP-9）蛋白表达水平的影响。结果：与空白组比较，辣椒碱（25，50，75，100 μmol·L-1）作用24 h后均对细胞存活率未产生明显影响，从150 μmol·L-1开始，随着辣椒碱浓度逐渐升高，细胞存活率逐渐下降（P<0.01），且呈剂量依赖效应；与空白组比较，辣椒碱（50，75，100 μmol·L-1）干预能显著抑制肝癌SMMC-7721细胞迁移和侵袭，且呈浓度依赖效应（P<0.01）；与空白组比较，辣椒碱（50，75，100 μmol·L-1）干预能明显上调肝癌SMMC-7721细胞中E-cadherin蛋白表达水平（P<0.01），下调Vimentin，MMP-2，MMP-9蛋白的表达水平（P<0.01）。结论：辣椒碱干预对肝癌SMMC-7721细胞迁移和侵袭具有抑制作用，可能与其上调肝癌SMMC-7721细胞中E-cadherin蛋白的表达，下调肝癌SMMC-7721细胞中Vimentin，MMP-2和MMP-9蛋白的表达有关。

Abstract

Objective: To investigate the effects of capsaicin on the migration and invasion of hepatoma SMMC-7721 cells and explore its possible molecular mechanism. Method: Different concentrations of capsaicin groups and blank group were established

and the cell viability was detected by cell counting kit-8 method 24 h after capsaicin (0

100

150

200

250 μmol·L-1) treatment on hepatoma SMMC-7721 cells. The effect of capsaicin (0

100 μmol·L-1) on cell migration and invasion ability of SMMC-7721 cells was detected by transwell. Western blot analysis was used to detect the protein expression levels of E-cadherin

Vimentin

matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) in cells treated with capsaicin(0

100 μmol·L-1)for 24 h. Result: As compared with the blank group

capsaicin(25

100 μmol·L-1)had no significant effect on cell survival rates after 24 h

and starting from 150 μmol·L-1

the cell survival rate was gradually decreased as the concentration of capsaicin was gradually increased (P<0.01)

showing a dose-dependent effect. As compared with the blank group

capsaicin(50

100 μmol·L-1)intervention significantly inhibited migration and invasion of hepatoma SMMC-7721 cells in a concentration-dependent manner (P<0.01). As compared with the blank control group

capsaicin(50

100 μmol·L-1)intervention significantly up-regulated the E-cadherin protein expression level in SMMC-7721 cells (P<0.01)

down-regulated Vimentin

MMP-2 and MMP-9 protein expression levels (P<0.01). Conclusion: Capsaicin can inhibit the migration and invasion of hepatoma SMMC-7721 cells

and the mechanism may be related to up-regulating the expression of E-cadherin and down-regulating the expressions of Vimentin

MMP-2 and MMP-9 in SMMC-7721 cells.

关键词

Keywords

references

Views

168

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Capsaicin on Proliferation and HMGB1 and IL-6 Expressions in Hepatoma SMMC-7721 Cells

Allicin Inhibits Epithelial-mesenchymal Transition of Human Pancreatic Cancer PANC-1 Cells

Effect of Morindae Officinalis Radix Processed with Different Proportions of Glycyrrhizae Radix et Rhizoma on Improvement of Renal Function and HPG Axis of Kidney Yang Deficiency Model Rats Induced by Adenine

Effect of Stemona tuberosa Alkaloids on Apoptosis of SMMC-7721 Cells and Expression of Bcl-2， Bax， and Cleaved Caspase-3

Capsaicin Induces Apoptosis of Colon Cancer SW480 Cells Through TRPV1

Related Author

Chan-chan XIAO

Mao-jian CHEN

Fan-biao MEI

Guan-hua REN

Tian-ren HUANG

Ji-lin LI

Wei DENG

HE Chuan

Related Institution

Affiliated Tumor Hospital of Guangxi Medical University

College of Pharmacy， Fujian University of Traditional Chinese Medicine

Zhengzhou University of Industrial Technology

Guangxi University of Chinese Medicine

Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine， Guangxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰