HU Hai-bo, HUANG Qiao-hua, HUANG Juan, et al. Surface Characters and Microscopic Observation of Wahlenbergiae Marginatae Radix seu Herba[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 46-51.
DOI:
HU Hai-bo, HUANG Qiao-hua, HUANG Juan, et al. Surface Characters and Microscopic Observation of Wahlenbergiae Marginatae Radix seu Herba[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 46-51. DOI: 10.13422/j.cnki.syfjx.20181804.
Surface Characters and Microscopic Observation of Wahlenbergiae Marginatae Radix seu Herba
Objective:To systematically study on the pharmacognosy characteristics of Wahlenbergiae Marginatae Radix seu Herba
in order to provide references for its identification
quality standards and further development and utilization. Method:Under Digital-Camera 6.0 software environment combined with digital microscope and electronic eyepiece
microscopic character observation was used to observe surface characteristics of the whole plant.Tissue sections were prepared by frozen section method
then microscopic observations
photographs
measurements
and related data were recorded.The superposition of depth of focus was treated by Adobe Bridge CS6 and Photoshop CS6 softwares. Result:Roots of Wahlenbergiae Marginatae Radix seu Herba had lots of laticifer groups and irregular-crystal inulins in cortex and phloem;its stems had many infinitive stomata in flat axis
collenchymas
single-cell non-glandular hairs
endodermis
single distributed laticifers and fiber bundles.The main identificational points in its leaves were squarish epidermal cells with cuticle
infinitive stomata
single-cell non-glandular hairs
collateral vascular bundle and obvious endothelium ring.And also there were lots of other points in the crude herb
such as protuberances in the petals margins
pollen morphology
the size of fruits and seeds
wrinkles and longitudinal stripes in the surface of seeds.Hence
the microscope characteristics of the powder of Wahlenbergiae Marginatae Radix seu Herba contained lots of multi-hole fibers
infinitive stomata in flat axis
single-cell type non-glandular hairs
inulins in irregular crystals;and also there were leaf epidermal cells of shallow waves
oblong seeds of longitudinal stripes
scattered colorless transparent oil drops
round pollen grains of three germinating holes in the medicinal powder;the above aspects could be used as the main identificational points of its powder. Conclusion:The main identificational points of Wahlenbergiae Marginatae Radix seu Herba include the characteristics of the transverse section of its roots
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