Simultaneous Determination of Seven Components in  by HPLC

SUN Zhi-wei; ZHANG Wen-jun; MA Ying-li

doi:10.13422/j.cnki.syfjx.20181810

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Seven Components in by HPLC

更新时间：2024-09-23

- Simultaneous Determination of Seven Components in by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 24, Pages: 78-82(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181810
  CLC： O657.72;R284.1
- Published：2018
- 稿件说明：
移动端阅览
SUN Zhi-wei, ZHANG Wen-jun, MA Ying-li. Simultaneous Determination of Seven Components in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(24): 78-82.
DOI：

SUN Zhi-wei, ZHANG Wen-jun, MA Ying-li. Simultaneous Determination of Seven Components in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(24): 78-82. DOI： 10.13422/j.cnki.syfjx.20181810.

摘要

目的：利用高效液相色谱配置二极管阵列检测器，旨在建立同时测定蓬子菜中鸢尾苷，saikoisoflavonoside A，槲皮素-3-O-β-D葡萄糖苷，槲皮素，香叶木素，芹菜素和5，4'-二羟基-3，6，7，8，3'-五甲氧基黄酮的方法。方法： Ultimate XB C18色谱柱（4.6 mm×250 mm，5 μm）；流动相甲醇-0.05%磷酸水溶液梯度洗脱；柱温35℃；流速1.0 mL·min-1；检测波长蓬子菜中鸢尾苷，saikoisoflavonoside A，槲皮素-3-O-β-D葡萄糖苷，槲皮素，香叶木素，芹菜素和5，4'-二羟基-3，6，7，8，3'-五甲氧基黄酮为260 nm。结果：蓬子菜中鸢尾苷，saikoisoflavonoside A，槲皮素-3-O-β-D葡萄糖苷，槲皮素，香叶木素，芹菜素和5，4'-二羟基-3，6，7，8，3'-五甲氧基黄酮分别在9.3~186 mg·L-1（r=0.999 4），12.8~256 mg·L-1（r=0.999 3），50~1 000 mg·L-1（r=0.999 3），38.7~774 mg·L-1（r=0.999 5），14.5~290 mg·L-1（r=0.999 2），27.4~548 mg·L-1（r=0.999 7）和8.6~172 mg·L-1（r=0.999 6）呈良好的线性关系，平均回收率分别为98.15%（RSD 2.4%），101.30%（RSD 2.2%），99.18%（RSD 1.1%），99.96%（RSD 1.4%），101.88%（RSD 0.9%），100.02%（RSD 2.3%）和101.71%（RSD 1.4%）。结论：该方法操作简单、结果准确，具有较好的重复性和稳定性，可用于同时测定蓬子菜乙酸乙酯有效部位中7种主要有效成分，将为蓬子菜质量控制提供科学依据。

Abstract

Objective: To establish the method for simultaneous determination of the contents of tectoridin

saikoisoflavonoside A

quercetin-3-O-glucopyranoside

quercetin

diosmetin

apigenin and 5

4'-dihydroxy-3

3'-pentamethoxy flavone based on high performance liquid chromatography diode array detection method of Calium verum. Method: Ultimate XB C18 column (4.6 mm×250 mm

5 μm) was used with methanol-0.05% phosphoric acid solution as the mobile phase for gradient elution

at a flow rate of 1.0 mL·min-1. The column temperature was 35℃. The determination wavelength was 260 nm for tectoridin

saikoisoflavonoside A

quercetin-3-O-glucopyranoside

quercetin

diosmetin

apigenin and 5

4'-dihydroxy-3

3'-pentamethoxy flavone. Result: There was a linear correlation within the range of 9.3-186 mg·L-1(r=0.999 4)

12.8-256 mg·L-1(r=0.999 3)

50-1 000 mg·L-1(r=0.999 3)

38.7-774 mg·L-1(r=0.999 5)

14.5-290 mg·L-1(r=0.999 2)

27.4-548 mg·L-1(r=0.999 7) and 8.6-172 mg·L-1(r=0.999 6) respectively for tectoridin

saikoisoflavonoside A

quercetin-3-O-glucopyranoside

quercetin

diosmetin and 5

4'-dihydroxy-3

3'-pentamethoxy flavones. The average recovery rate was 98.15%(RSD 2.4%)

101.30%(RSD 2.2%)

99.18%(RSD 1.1%)

99.96%(RSD 1.4%)

101.88%(RSD 0.9%)

100.02%(RSD 2.3%) and 101.71%(RSD 1.4%)respectively for tectoridin

saikoisoflavonoside A

quercetin-3-O-glucopyranoside

quercetin

diosmetin

apigenin and 5

4'-dihydroxy-3

3'-pentamethoxy flavone. Conclusion: The analysis method is simple

rapid

reproducible

accurate

and reliable

and can be used to identify and determine the contents of seven constituents in G. verum

providing scientific basis for its quality control.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Optimization of Extraction Technology for Main Components from Flower of

Quercetin Attenuates Ferroptosis Against LPS-induced Acute Kidney Injury Rats via Modulating Keap1/Nrf2/ARE Pathway

Effect of Quercetin on Cuproptosis in Rheumatoid Arthritis Rats and Its Mechanism via SLC31A1/FDX1 Pathway

Apigenin Induces Apoptosis of Human Colorectal Cancer CL187 Cells via PI3K/Akt and MAPK Signaling Pathways

Quercetin Against Cerebral Ischemia-reperfusion Injury： A Review

Related Author

LUO Chong

SUN Hui

CHEN Fang

LAI Jia-ping

YANG Haoruo

YU Dajun

ZHANG Yu

YANG Bin

Related Institution

Xiyuan Hospital， China Academy of Chinese Medical Sciences

Tianjin University of Chinese Medicine

Tianjin University of Traditional Chinese Medicine

Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine， Guangxi University of Chinese Medicine

Guangxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰