Content Determination of Ten Kinds of Stilbene Glycosides in Polygoni Multiflori Radix by UPLC-MS/MS

YU Yi; GAO Feng; LI Jia-xing; JIN Yan; MA Fang-li; YANG Jian; GUO Lan-ping

doi:10.13422/j.cnki.syfjx.20181811

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Content Determination of Ten Kinds of Stilbene Glycosides in Polygoni Multiflori Radix by UPLC-MS/MS

更新时间：2024-09-23

- Content Determination of Ten Kinds of Stilbene Glycosides in Polygoni Multiflori Radix by UPLC-MS/MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 23, Pages: 87-92(2018)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.20181811
  CLC： R284.1
- Published：2018
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YU Yi, GAO Feng, LI Jia-xing, et al. Content Determination of Ten Kinds of Stilbene Glycosides in Polygoni Multiflori Radix by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 87-92.
DOI：

YU Yi, GAO Feng, LI Jia-xing, et al. Content Determination of Ten Kinds of Stilbene Glycosides in Polygoni Multiflori Radix by UPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(23): 87-92. DOI： 10.13422/j.cnki.syfjx.20181811.

摘要

目的：建立超高效液相色谱三重四级杆串联质谱法同时测定不同产地何首乌中10种二苯乙烯苷类成分的方法，比较不同产地何首乌该类成分的含量差异。方法：采用ACQUITY UPLC BEH C18色谱柱（2.1 mm×100 mm，1.8 μm）；以0.1%甲酸水（A）-0.1%甲酸乙腈（B）溶液为流动相，梯度洗脱，流速0.5 mL·min-1，柱温40℃；采用电喷雾离子源，负离子检测方式，得到相应的提取离子流图，以峰面积进行定量。结果：何首乌中白藜芦醇，虎杖苷，2，3，5，4'-四羟基二苯乙烯-2-O-葡萄糖苷，2，3，5，4'-四羟基二苯乙烯-2，3-O-二葡萄糖苷，2，3，5，4'-四羟基二苯乙烯-2-O-葡萄糖基-（1→6）-葡萄糖苷，2，3，5，4'-四羟基二苯乙烯-2-O-（2″-O-没食子酰基）-葡萄糖苷，2，3，5，4'-四羟基二苯乙烯-2-O-（6″-O-乙酰基）-葡萄糖苷，2，3，5，4'-四羟基二苯乙烯-2-O-（2″-O-阿魏酰基）-葡萄糖苷和2，3，5，4'-四羟基二苯乙烯-2-O-（2″-O-香豆酰基）-葡萄糖苷具有良好的线性关系，r均≥0.998 7，平均加样回收率为96.5%~106.3%，RSD均≤3.5%。结论：该方法操作简便、准确、重复性好，为何首乌二苯乙烯苷类成分的深入研究及其质量控制提供了参考依据。

Abstract

Objective:To establish a ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS/MS) method for the simultaneous determination of ten kinds of stilbene glycosides in Polygoni Multiflori Radix praeparata

and compare their contents in samples from different origins. Method:The determination was performed on the ACQUITY UPLC BEH C18(2.1 mm×100 mm

1.8 μm);with 0.1% formic acid water(A) and 0.1% formic acid acetonitrile(B) as the mobile phase for gradient elution. The flow rate was 0.5 mL·min-1and the column temperature was 40℃. The extracted ion chromatograms were obtained with ESI- ion source under negative ion detection method

and the peak area was used for quantitative analysis. Result:The resveratrol

polydatin

4'-tetrahydroxysilbene-2-O-glucopyranoside

4'-tetrahydroxysilbene-2

3-di-O-glucopyranoside

4'-tetrahydroxysilbene-2-O-glucopyransyl-(1→6)-glucopyranoside

4'-tetrahydroxysilbene-2-O-(2″-O-galloyl)-glucopyranoside

4'-tetrahydroxystilbene-2-O-(6″-O-acetyl)-glucopyranoside

4'-tetrahydroxy silbene-2-O-(2″-O-ferulicacyl)-glucopyranoside

4'-tetrahydroxysilbene-2-O-(2″-O-coumaryl)-glucopyranoside and 2

4'-tetrahydroxystilbene-2-O-(2″-O-acetyl)-glucopyranoside in Polygoni Multiflori Radix showed good linearity relationship

r ≥ 0.998 7.The average recovery rate was 96.5%-106.3%

RSD ≤ 3.5%. Conclusion:The established method is simple

accurate and reproducible for the determination and quality control of Polygoni Multiflori Radix.

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