Effect of Capsaicin on Proliferation of Human Breast Cancer MCF-7 Cells and p21 and FBI-1 Expression

CHEN Mao-jian; YANG Wei-ping; QIN Qing-hong; XIAO Chan-chan; JIANG Wei; LI Hui-min; WEI Chang-yuan

doi:10.13422/j.cnki.syfjx.20181816

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Effect of Capsaicin on Proliferation of Human Breast Cancer MCF-7 Cells and p21 and FBI-1 Expression

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- Effect of Capsaicin on Proliferation of Human Breast Cancer MCF-7 Cells and p21 and FBI-1 Expression
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 22, Pages: 102-106(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181816
  CLC： R285
- Published：2018
- 稿件说明：
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CHEN Mao-jian, YANG Wei-ping, QIN Qing-hong, et al. Effect of Capsaicin on Proliferation of Human Breast Cancer MCF-7 Cells and p21 and FBI-1 Expression[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 102-106.
DOI：

CHEN Mao-jian, YANG Wei-ping, QIN Qing-hong, et al. Effect of Capsaicin on Proliferation of Human Breast Cancer MCF-7 Cells and p21 and FBI-1 Expression[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(22): 102-106. DOI： 10.13422/j.cnki.syfjx.20181816.

摘要

目的：观察辣椒碱对人乳腺癌MCF-7细胞增殖的影响及可能的分子机制。方法：设立辣椒碱组以及空白组，采用细胞计数试剂盒-8法（CCK-8）检测辣椒碱（50，100，150，200，250，300 μmol·L-1）处理MCF-7细胞24，48，72 h后的细胞活性；克隆形成实验检测辣椒碱（100，150，200 μmol·L-1）处理MCF-7细胞18 h再更换为完全培养基继续培养14 d后的克隆形成能力；实时荧光定量PCR（Real-time PCR）检测辣椒碱（100，150，200 μmol·L-1）处理MCF-7细胞24 h后细胞中细胞周期依赖性蛋白激酶抑制因子p21（p21）和人类免疫缺陷病毒短转录诱导物连接因子-1（FBI-1）mRNA表达水平；蛋白质免疫印迹法（Western blot）检测辣椒碱（100，150，200 μmol·L-1）处理MCF-7细胞24 h后细胞中p21和FBI-1蛋白的表达水平。结果：与空白组比较，辣椒碱（50，100，150，200，250，300 μmol·L-1）在体外对MCF-7细胞活性具有明显的抑制作用（P<0.05，P<0.01），并且这种抑制作用呈浓度和时间依赖效应；与空白组比较，辣椒碱（100，150，200 μmol·L-1）均能显著抑制MCF-7细胞的克隆形成能力（P<0.01）；与空白组比较，辣椒碱（100，150，200 μmol·L-1）能显著上调MCF-7细胞中p21 mRNA和蛋白的表达水平（P<0.01），下调FBI-1 mRNA和蛋白的表达水平（P<0.01）。结论：辣椒碱干预在体外对乳腺癌MCF-7细胞增殖具有抑制作用，潜在机制可能与其上调细胞中p21 mRNA和蛋白的表达水平，下调细胞中FBI-1 mRNA和蛋白的表达水平有关。

Abstract

Objective:To investigate the effect of capsaicin on proliferation of human breast cancer MCF-7 cells and its possible molecular mechanism. Method:Capsaicin groups with different concentrations and blank group were set up. The cell viability was detected by cell count kit-8 (CCK-8) assay after MCF-7 cells were treated with capsaicin (50

100

150

200

250

300 μmol·L-1) for 24

72 h. The cell colony formation ability was verified by colony formation assay after MCF-7 cells were treated with capsaicin(100

150

200 μmol·L-1) for 18 h and then incubated in complete medium for 14 days. The mRNA expression levels of cyclin-dependent kinase inhibitor p21 and factor that binds to the inducer of short transcripts of human immunodeficiency virus-1 (FBI-1) were measured by Real-time polymerase chain reaction (Real-time PCR) after MCF-7 cells were treated with capsaicin (100

150

200 μmol·L-1) for 24 h. The protein expression levels of p21 and FBI-1 were measured by Western blot after MCF-7 cells were treated with capsaicin (100

150

200 μmol·L-1) for 24 h. Result:As compared with the blank group

capsaicin (50

100

150

200

250

300 μmol·L-1) significantly inhibited the cell viability of MCF-7 cells (P<0.05

P<0.01) in dose and time dependent manners. Capsaicin (100

150

200 μmol·L-1) also markedly inhibited the cell colony formation ability (P<0.01). In addition

capsaicin (100

150

200 μmol·L-1) up-regulated the mRNA and protein expressions of p21 and down-regulated mRNA and protein expressions of FBI-1 (P<0.01). Conclusion:Capsaicin inhibits cell proliferation of MCF-7 cells

and the possible mechanism may be related to up-regulation of p21 and down-regulation of FBI-1 at both mRNA and protein levels.

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