Objective:To separate the chemical constituents of n-butanol and dichloromethane ether fractions from Polygonatum cyrtonema and identify the structures of the compounds. Method:Rhizomes of P. cyrtonema were dried and crushed at 60℃ saccording to the traditional processing method of Jiangxi Jianchang

soaked by 70% ethanol for 1 hour

extracted for 2 times

filtered

and then extracted by three times with the two times volume of dichloromethane

ethyl acetate and n -butanol respectively. The extraction solution was decompressed and concentrated to obtain the dichloromethane fraction

ethyl acetate fraction and n -butanol fraction. The n -butanol and dichloromethane ether fractions from P. cyrtonema were isolated by macroporous adsorption resin chromatography

silica gelcolumn

re-crystallization

Sephadex LH-20 column

reversed phase high-performance liquid chromatography and their structures were identified according to physicochemical properties and spectral evidences. Result:Fourteen compounds were isolated from n -butanol and dichloromethane ether fractions of Polygonatum cyrtonema and elucidated as (25 S)spirostan-5-en-12-one-3-O-D -glucopyranosyl-(1→2)-O -[β-D -xylopyranosyl(1→3)]-O-β-D -glucopyranosyl(1→4)-β-D -galactopyranoside(1)

(25 S)spirostan-5-en-12-one-3-O-β-D -glucopyranosyl-(1→2)-O -[β-D -glucopyranosyl-(1→3)]-O-β-D -glucopyranosyl-(1→4)-β-D -galactopyranoside(2)

dibutyl phthalate(3)

daucosterol(4)

β -Sitosterol(5)

hypofuran B(6)

(25 R)-3-β-hydroxyspirost-5-en-12-one(7)

(+)-medioresinol(8)

polygonatine A(9)

5-hydroxymethyl furfural(10)

5-hydroxy maltol(11)

2-(4-hydroxyphenyl)-ethanol(12)

1H-indole-3-carboxaldehyde(13)

and polygonatine B(14). Conclusion:Compounds 4

5

9 and 14 were firstly obtained from P. cyrtonema

and compounds 6

8

12 and 13 were isolated from the plants of Polygonatum for the first time

providing chemical basis and foundation for the comprehensive development and the research on active substances of natural plant.