YANG Fan, LIU Yu-xia, LI Jiong, et al. Effect of Branch Blight on Yield and Quality of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 32-37.
DOI:
YANG Fan, LIU Yu-xia, LI Jiong, et al. Effect of Branch Blight on Yield and Quality of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(20): 32-37. DOI: 10.13422/j.cnki.syfjx.20181914.
Objective: To investigate the effect of branch blight on the yield of honeysuckle as well as the yield and the content of active components of Lonicera japonica
and provide scientific basis for field management on branch blight disease and quality evaluation.Method: The yield of honeysuckle was measured by the number of buds and their total weight harvested at the first full-bloom stage.The contents of chlorogenic acid and luteolin-7-O-glucosid in honeysuckle samples andthe contents of chlorogenic acid and loganin in the L. japonica caulis from all grades of L. japonica were measured according to method illustrated in Chinese Pharmacopoeia (2015).Result: The average number
fresh weight and dry weight of buds per plant from grade 0 grade 7 were significantly different (P<0.01). Among them
healthy plants had the highest quantity of buds and grade 7 plant had the lowest quantity. The higher the disease grade
the smaller the amount of buds and the lower the yield. The contents of active components including chlorogenic acid and luteolin-7-O -glucosid in honeysuckle samples andchlorogenic acid and loganin in the L. japonica caulis from all disease grades were in line with the standards in Chinese Pharmacopoeia (2015). The content of chlorogenic acid in all honeysuckle samples ranged from 2.31% to 3.46%
among which the content of chlorogenic acid in the honeysuckle sample from grade 1 of diseased tree was the highest
while that from grade 0 (healthy plant) was the lowest. The content of chlorogenic acid in the samples from diseased tree was significantly higher than that from healthy plants (P<0.01).The content of luteolin-7-O -glucosid in honeysuckle samples ranged from 0.050% to 0.065%
among which the content of luteolin-7-O -glucosid from the grade 0 honeysuckle sample was the lowest. The content of luteolin-7-O -glucosid in healthy plants was significantly different from other disease grades (P<0.01). The content of chlorogenic acid in L. japonica caulis was 0.43% to 0.54%
and the content of chlorogenic acid in healthy plants was higher than that in other disease-grade plants (P <0.01). The content of loganin in L. japonica caulis was 0.82% to 1.58%
and the content of loganin in all diseased sample plants of L. japonica caulis was significantly lower than that in healthy ones(P <0.01).Conclusion: The branch blight disease was beneficial to the accumulation of chlorogenic acid and Luteolin in honeysuckle
but could significantly decrease the yield and the contents of chlorogenic acid and loganin in the L. japonica caulis.