Identification of Chemical Constituents from Ethyl Acetate Extract of

HOU Yuan-xin; ZHOU Chang-yuan; HOU Shu-fen; GAO Rong-min

doi:10.13422/j.cnki.syfjx.20181915

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Identification of Chemical Constituents from Ethyl Acetate Extract of

更新时间：2024-01-04

- Identification of Chemical Constituents from Ethyl Acetate Extract of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 24, Issue 19, Pages: 52-55(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20181915
  CLC： R284.1
- Published：2018
- 稿件说明：
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HOU Yuan-xin, ZHOU Chang-yuan, HOU Shu-fen, et al. Identification of Chemical Constituents from Ethyl Acetate Extract of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 52-55.
DOI：

HOU Yuan-xin, ZHOU Chang-yuan, HOU Shu-fen, et al. Identification of Chemical Constituents from Ethyl Acetate Extract of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(19): 52-55. DOI： 10.13422/j.cnki.syfjx.20181915.

摘要

目的：对蜈蚣草Pteris vittata乙酸乙酯部位进行系统的化学成分研究。方法：蜈蚣草用95%乙醇回流提取3次，提取液减压浓缩成浸膏后分散于水中，然后依次用石油醚、乙酸乙酯和正丁醇进行萃取，回收溶剂后得到其石油醚、乙酸乙酯和正丁醇部位萃取物。对乙酸乙酯部位萃取物综合应用硅胶柱色谱，Sephadex LH-20凝胶柱色谱，ODS柱色谱和重结晶等技术分离纯化，得到单体化合物，并结合理化性质、核磁共振谱、质谱数据和相关文献鉴定单体化合物的结构。结果：从蜈蚣草乙酸乙酯部位萃取物中共分离得到12个化合物，分别鉴定结构为芹菜素（1），木犀草素（2），木犀草素-7，3'，4'-三甲醚（3），3'-甲氧基木犀草素（4），7-羟基-8-甲氧基香豆素（5），芹菜素-7-O-β-D-葡萄糖醛酸（6），木犀草素-7-O-β-D-吡喃葡萄糖苷（7），柯伊利素-7-O-β-D-吡喃葡萄糖苷（8），紫云英苷（9），对羟基苯甲酸（10），β-谷甾醇（11），β-胡萝卜苷（12）。结论：化合物2~5和8~12为首次从该植物中分离得到。该化学成分研究为蜈蚣草的临床应用及药理活性研究提供了资料参考。

Abstract

Objective: To study the chemical constituents from the ethyl acetate extract of Pteris vittata. Method: The whole plant of P. vittata was powdered and extracted with 95% EtOH for 3 times under reflux. After removal of the solvent

the 95% EtOH residue was suspended in water and successively extracted with petroleum ether

EtOAc and n-BuOH to obtain petroleum ether

EtOAc and n-BuOH extract respectively. The EtOAc extract was isolated and purified by silica gel

Sephadex LH-20

ODS columns and recrystallization

and the structures of their monomeric compounds were identified on the basis of physicochemical properties

NMR spectra

mass spectrum analysis and related literature. Result: Twelve compounds were isolated from the ethyl acetate extract and identified as apigenin(1)

luteolin(2)

luteolin-7

4'-trimethyl ether(3)

chrysoeriol(4)

7-hydroxy-8-methoxycoumarin(5)

apigenin-7-O-β-D-glucuronide(6)

luteolin-7-O-β-D-glucopyranoside(7)

chrysoeriol-7-O-β-D-glucopyranoside(8)

astragalin(9)

p-hydroxybenzoic acid(10)

β-sitosterol(11) and β-daucosterol(12). Conclusion: Compounds 2-5 and 8-12 were firstly isolated from P. vittata

providing reference for its clinical application and pharmacological studies.

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Related Institution

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